Anti-SAE1 Antibody,

Also for SAE1 (NM_005500)

OriGene Data

Immunogen	This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human SAE1.
Clone Name		Isotype	Rabbit Ig
Species Reactivity	Human	Concentration	0.25 mg/ml
Guaranteed Application *	WB, IHC	Suggested Dilutions	WB: 1: 100~500; IHC: 1:50~100; ELISA: 1: 1,000;
Buffer	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Purification	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. (Protein A or G Sepharose)

Reference Data

Target Name	Homo sapiens SUMO1 activating enzyme subunit 1 (SAE1), transcript variant 1
Alternative Name	AOS1; HSPC140; SUA1; UBLE1A
Database Link	NP_005491
Function	Covalent attachment of one protein to another is one of the more prominent posttranslational modifications in respects to size and ubiquity ? to which eukaryotic proteins are subject. Ubiquitin is the most familiar of the protein modifiers and its activation and transfer to target proteins has been studied for over two decades. Recently a new group of ubiquitin-like (Ubl) proteins have come to light. One of the most intriguing of them is SUMO (small ubiquitin-like modifier, ~12kDa) also known as Sentrin. SUMO family has been described in vertebrates: SUMO-1 and the closest homologs SUMO-2 and SUMO-3. SUMO have been shown to bind and regulate mammalian SP-RINGs (such as Mdm2, PIAS and PML), RanGAP1, RanBP2, p53, p73, HIPK2, TEL, c-Jun, Fas, Daxx, TNFRI, Topo-I, Topo-II, WRN, Sp100, IkB-alpha, Androgen receptor (AR), GLUT1/4, Drosophila Ttk69, Dorsal, CaMK, yeast Septins, and viral CMV-IE1/2, EBV-BZLF1, HPV/BPV-E1. These bindings implicate SUMO in the stabilization of the target proteins and/or their localization to subcellular complexes. SUMO research enters now an exciting phase with a promise to help understanding how cells orchestrate the complexities of rapidly regulating protein level and activity.
Related Pathway

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WB Image

(LEFT) The anti-SAE1 N-term Pab is used in Western blot to detect SAE1 in HL60 cell lysate. (RIGHT) Western blot analysis of SAE1 (arrow) using rabbit polyclonal SAE1 Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SAE1 gene (Lane 2) (Origene Technologies).

IHC Image

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Conjugation Services
OriGene provides conjugation services of TrueMAB antibodies with the following molecules:

HRP
Biotin
PE
FITC
DyLight® 488
DyLight® 550
DyLight® 594
DyLight® 633

* DyLight is a trademark of Thermo-Fisher Scientific

Bulk Order
OriGene offers bulk sizes of TrueMAB antibodies in both the BSA/glycerol formulation and the carrier-free (BSA/glycerol-free) formulation.

Anti-SAE1 Antibody

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

OriGene Data

Reference Data