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Anti-S100A10 Antibody EPR3318
Also for S100A10 (NM_002966)
|A synthetic peptide corresponding to residues in human Calpactin I Light Chain/S100A10 was used as an immunogen.|
||Tissue culture supernatant
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, FC
||WB: 1:1000 - 1:10000; IP: 1:50; FC: 1:100; IHC-P: 1:100 - 1:250
|Does not react with Mouse, Rat. Is unsuitable for ICC.|
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA (Protein A or G Sepharose)
|Is unsuitable for ICC.
|Homo sapiens S100 calcium binding protein A10 (S100A10)|
|42C; ANX2L; ANX2LG; CAL1L; Ca; CLP11; GP11; p10; P11|
|S100 proteins are low-molecular-weight calcium-binding proteins of the EF-hand superfamily and appear to be involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation (1). Also, S100 proteins have been reported to localize to the plasma membrane in differentiated keratinocytes, suggesting a role in regulating calcium-dependent, membrane-associated events (3). The Calpactin I light chain (S100 calcium-binding protein A10; S100A10) is a member of the S100 family and it forms a heterotetrameric complex with another light chain, p11, and two heavy chains, annexin II and p36 (2). S100A10 is present in basal and spinous cells, in the cytoplasm, and is associated with the plasma membrane. S100A10, as well as a few other members of the S100 family, are suggested as transglutaminase substrates (3). |
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Western blot - Anti-S100A10 antibody [EPR3318]; All lanes : Anti-S100A10 antibody [EPR3318] at 1/1000 dilution.Lane 1 : HACAT cell lysate.Lane 2 : HT29 cell lysate.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 11 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A10 antibody [EPR3318]; Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labelling S100A10 with ab134176 at 1/100 dilution.
Flow Cytometry - Anti-S100A10 antibody [EPR3318]; ab134176 staining S100A10 in the HT-29cell line by Flow Cytometry(red). Rabbit IgG was used as a negative control (green).