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Anti-S100A10 Antibody EPR3317
Also for S100A10 (NM_002966)
|A synthetic peptide corresponding to residues in human Calpactin I light chain/S100A10 was used as an immunogen.|
||Tissue culture supernatant
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, FC
||WB: 1:5000 - 1:20000; IHC-P: 1:100 - 1:250; ICC: 1:50 - 1:100; FC: 1:50
|Does not react with Mouse, Rat. Is unsuitable for IP.|
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05% (Protein A or G Sepharose)
|Is unsuitable for IP.
|Homo sapiens S100 calcium binding protein A10 (S100A10)|
|42C; ANX2L; ANX2LG; CAL1L; Ca; CLP11; GP11; p10; P11|
|S100 proteins are low-molecular-weight calcium-binding proteins of the EF-hand superfamily and appear to be involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation (1). The Calpactin I light chain (S100 calcium-binding protein A10; S100A10) is a member of the S100 family and it forms a heterotetrameric complex with another calcium-binding protein, annexin II(2). S100A10 is present in basal and spinous cells, in the cytoplasm, and is associated with the plasma membrane (3). The basic unit for S100A10 is a tight, non-covalent dimmer (4). S100A10, as well as a few other members of the S100 family, are suggested as transglutaminase substrates. Also, S100 proteins have been reported to localize to the plasma membrane in differentiated keratinocytes, suggesting a role in regulating calcium-dependent, membrane-associated events (3). |
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Western blot - S100A10 antibody [EPR3317]; All lanes : Anti-S100A10 antibody [EPR3317] at 1/10000 dilution.Lane 1 : HaCaT cell lysate.Lane 2 : A431 cell lysate.Lane 3 : HT-29 cell lysate.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit at 1/1000 dilution.Predicted band size : 11 kDa.Observed band size : 11 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - S100A10 antibody [EPR3317]; ab76472 at 1/100 dilution staining S100A10 in human colon tissue.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - S100A10 antibody [EPR3317]; ab76472 at 1/100 dilution staining S100A10 in human kidney tissue.
Flow Cytometry - Anti-S100A10 antibody [EPR3317]; ab76472 stainingS100A10 inA431 cell line by Flow Cytometry(red). Rabbit IgG was used as a negative control (green).