RUNX2 Rabbit Polyclonal Antibody

CAT#: TA309842

Rabbit Polyclonal RUNX2 Antibody

Product Images

Immunocytochemistry/Immunofluorescence: RUNX2/CBFA1 Antibody TA309842 - RUNX2 antibody was tested in HeLa cells with FITC (green). Nuclei and alpha-tubulin were counterstained with Dapi (blue) and Dylight 550 (red).

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  Flow Cytometry: RUNX2/CBFA1 Antibody TA309842 - An intracellular stain was performed on HeLa cells with RUNX2/CBFA1 Antibody TA309842AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.

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  Immunohistochemistry: RUNX2/CBFA1 Antibody TA309842 - Staining of RUNX2 in mouse prostate using DAB with hematoxylin counterstain.

Specifications

Product Data

• Applications: FC, ICC/IF, IHC
• Recommended Dilution: Immunohistochemistry-Paraffin: 1:200, Immunohistochemistry: 1:200, Immunocytochemistry/ Immunofluorescence: 1:50-1:100, Flow Cytometry: 2-5 ug/ml
• Reactivities: Human, Mouse
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: A synthetic peptide made to an internal region of the human RUNX2 protein (within residues 225-300). [Swiss-Prot Q13950]
• Formulation: PBS, 30% glycerol and 0.05% Sodium Azide
• Concentration: lot specific
• Purification: Affinity purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: runt related transcription factor 2
• Database Link:
  NP_004339
  Entrez Gene 12393 MouseEntrez Gene 860 Human
  UniProt ID Q13950
• Synonyms: AML3; CBFA1; CCD; CCD1; OSF-2; OSF2; PEA2aA; PEBP2A1; PEBP2A2; PEBP2aA; PEBP2aA1

Reference Data

• Protein Families: Druggable Genome, Transcription Factors