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Home Antibody All anti-RPS6KA5 antibodies

Anti-RPS6KA5 Antibody E375

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA300536
  • Rabbit Monoclonal Antibody against RPS6KA5 (Clone E375)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC403633) , 20ug Explanation
100ul 325 3-7 Days
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WB(1)
IHC(1)
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Also for RPS6KA5 (NM_182398)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic phospho-peptide corresponding to residues surrounding Serine 376 of human MSK1 was used as immunogen. The antibody will detect MSK1 phosphorylation on Serine 376.
Clone NameE375 IsotypeIgG
Species ReactivityRat, Human (Does not react with: Mouse) ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC Suggested DilutionsWB: 1:1000 - 1:5000; ICC: 1:100 - 1:250; IP: 1:30; IHC-P: Use at an assay dependent dilution
BufferDoes not react with Mouse. Is unsuitable for Flow Cyt.
Purification PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Note Is unsuitable for Flow Cyt.

Reference Data

Target NameHomo sapiens ribosomal protein S6 kinase, 90kDa, polypeptide 5 (RPS6KA5), transcript variant 2
Alternative NameMSK1; MSPK1; RLPK
Database LinkNP_872198
Entrez Gene 9252 Human
Entrez Gene 314384 Rat
FunctionMSK-1 is a mitogen and stress activated protein kinase-1 which belongs to the AGC family of kinases and is related in structure to the ribosomal p70 S6 kinase subfamily. MSK-1can be activated by ERK1/2 and SAPK2 /p38 MAP kinase. It is also known to be required for the phosphorylation of CREB, ATF1 H3 and HMG-14 in response to mitogen and stress (1-2). Similar to RSK, MSK-1 contains two kinase domains (N-term and a C-term) (3). Once phosphorylated on Thr581 and Ser360 by ERK1/2 and SAPK2/p38, MSK-1 autophosphorylate on at least 5 sites. Of these autophosphorylation sites Ser212 and Ser376 get phosphorylated by the C-terminal kinase domain of MSK-1 which is essential for the catalytic activity of the N-terminal kinase domain (4).
Related PathwayProtein KinaseTranscription FactorsDruggable Genome MAPK signaling pathwayNeurotrophin signaling pathwayBladder cancer

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - MSK1 (phospho S376) antibody [E375]; All lanes : Anti-MSK1 (phospho S376) antibody [E375] at 1/5000 dilution.Lane 1 : Untreated K562 cell lysate.Lane 2 : K562 cell lysate treated with Alkaline.Predicted band size : 90 kDa.Observed band size : 87 kDa .
IHC Image
Immunohistochemistry (Paraffin-embedded sections) - MSK1 (phospho S376) antibody [E375]; Ab32190, at a dilution of 1/50, staining MSK1 in paraffin embedded human urinary bladder carcinoma tissue by Immunohistochemistry.

 

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