A phospho-specific peptide corresponding to residues surrounding Serine 227 of human RSK2 was used as an immunogen. The antibody only detects RSK2 phosphorylated on Serine 227.
Buffer
50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Mutations in this gene have been associated with Coffin-Lowry syndrome (CLS). [provided by RefSeq].
Related Pathway
EGFR1 Signaling Pathway
MAPK signaling pathway
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Western blot analysis on HeLa cell lysates using anti-Phospho-RSK2 (pS227) RabMAb (TA303693), 1:2000 dilution. Cells were either (A) untreated (B) treated with calyculin A
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