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Anti-RPE Antibody EPR7464
|A synthetic peptide corresponding to residues on the C-terminus in human RPE was used as an immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:1000; IP: 1:10 - 1:100
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
|Tissue culture supernatant (Protein A or G Sepharose)
|Is unsuitable for Flow Cyt,ICC or IHC-P.
|Homo sapiens ribulose-5-phosphate-3-epimerase (RPE), transcript variant 2|
|RPE is an iron enzyme with a mononuclear metal atom that binds the carbonyl and carboxylate moieties of ribulose-5-phosphate, facilitating epimerization by stabilizing the deprotonated anionic intermediate. It employs a ferrous iron atom as a solvent-exposed cofactor, and hydrogen peroxide (H2O2) rapidly oxidizes this metal in a Fenton reaction. Thus, RPE is rapidly damaged by H2O2, similar to Fe–S dehydratases (1).|
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Western blot - Anti-RPE antibody [EPR7464]; All lanes : Anti-RPE antibody [EPR7464] at 1/1000 dilution.Lane 1 : K562 cell lysate.Lane 2 : HeLa cell lysate.Lane 3 : HepG2 cell lysate.Lane 4 : 293T cell lysate.Lysates/proteins at 10 µg per lane.Secondary.HRP-conjugated goat anti-rabbit at 1/2000 dilution.Predicted band size : 25 kDa.