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Anti-RPA2 TrueMAB Antibody clone 10G1
TrueMAB Antibodies - Made against Authentic Protein Antigens
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Also for RPA2 (NM_002946)
|Full length human recombinant protein of human RPA2 (NP_002937) produced in HEK293T cell.|
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IP, FC
||WB 1:4000, IHC 1:50, FLOW 1:100, IP 2ug/500ul
|PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography (Protein A or G Sepharose)
|Homo sapiens replication protein A2, 32kDa (RPA2)|
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RPA2 (RC205715, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RPA2.
Immunohistochemical staining of paraffin-embedded Human liver tissue using anti-RPA2 mouse monoclonal antibody. (TA500762)
Immunohistochemical staining of paraffin-embedded Human Ovary tissue using anti-RPA2 mouse monoclonal antibody. (TA500762)
Immunoprecipitation(IP) of RPA2 by using TrueMab monoclonal anti-RPA2 antibodies (Negative control: IP without adding anti-RPA2 antibody.). For each experiment, 500ul of DDK tagged RPA2 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-RPA2 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
HEK293T cells transfected with either RC205715 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-RPA2 antibody(TA500762), and then analyzed by flow cytometry.