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Anti-ROCK2 Antibody EPR7141(B)
Also for ROCK2 (NM_004850)
|A synthetic peptide corresponding to residues at the C-terminus of human ROCK2 was used as an immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
|WB, ASSAY, IHC
||WB: 1:10000 - 1:50000; IHC-P: 1:50 - 1:100; ICC/IF: 1:100 - 1:250
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
|Tissue culture supernatant (Protein A or G Sepharose)
|Is unsuitable for Flow Cyt or IP.
|Homo sapiens Rho-associated, coiled-coil containing protein kinase 2 (ROCK2)|
|ROCK2 belongs to the protein kinase superfamily, and it is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. This protein, which is an isozyme of ROCK1, is a target for the small GTPase Rho (1,2). |
Signaling by GPCR
TGF Beta Signaling Pathway
Wnt Signaling Pathway
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Western blot - Anti-ROCK2 antibody [EPR7141(B)]; All lanes : Anti-ROCK2 antibody [EPR7141(B)] at 1/10000 dilution.Lane 1 : Hela cell lysate.Lane 2 : L6 cell lysate.Lane 3 : A673 cell lysate.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 161 kDa.
Other-Anti-ROCK2 antibody [EPR7141(B)](ab125025); Equilibrium disassociation constant (KD)..
Immunohistochemistry (Frozen sections) - Anti-ROCK2 antibody [EPR7141(B)]; ab125025 stainingROCK2 in Mouse hippocamus brain tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde and blocked with 10%serum for 60 minutes at 25°C. Samples were incubated with primary antibody (1/200 in PBS + 10% Goat serum + 2% Horse serum + 1% BSA) for 16 hours at 4°C. An Alexa Fluor?488-conjugatedGoat anti-rabbit polyclonal (1/1000) was used as the secondary antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROCK2 antibody [EPR7141(B)]; ab125025, at 1/50 dilution, staining ROCK2 in Paraffin-embedded Human kidney tissue by Immunohistochemistry.