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Anti-ROCK1 Antibody EP786Y
Also for ROCK1 (NM_005406)
|A synthetic peptide corresponding to residues near C-terminus of human ROCK1 was used as an immunogen. This antibody recognizes both the cleaved C-terminus of ROCK-1 (30 kDa) and full length protein.|
|Mouse, Rat, Cow, Human
||Lot dependent; please refer to CoA along with shipment
|WB, IHC, IF, FC
||IHC-Fr: 1:100; WB: 1:500; IHC-P: 1:50 - 1:100; ICC/IF: 1:250 - 1:500; IP: 1:50; FC: 1:1000
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant
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Western blot - ROCK1 antibody [EP786Y]; All lanes : Anti-ROCK1 antibody [EP786Y] at 1/500 dilution.Lane 1 : Untreated HeLa cell lysate.Lane 2 : Camptothecin treated HeLa lysate.Predicted band size : 158 kDa.Observed band size : 30,158 kDa .
Immunohistochemistry (Paraffin-embedded sections) - ROCK1 antibody [EP786Y]; Ab45171 staining human ROCK1 in human thyroid gland carcinoma by immunohistochemistry using paraffin embedded tissue.
Immunocytochemistry/ Immunofluorescence - ROCK1 antibody [EP786Y]; Immunofluorescent staining of HeLa cells using TA303688.
Flow Cytometry - Anti-ROCK1 antibody [EP786Y]; Overlay histogram showing HeLa cells stained with TA303688 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was Alexa Fluor 488 goat anti-rabbit IgG (H+L) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1ug/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.