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Home Antibody All anti-RNF25 antibodies

Anti-RNF25 Antibody

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA319495
  • Rabbit polyclonal anti-RNF25 antibody
  • FREE positive control: HEK293T cell transient overexpression lysate (LC411668) , 20ug
100ug 325 3-7 Days
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WB(1)
IP(1)
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Also for RNF25 (NM_022453)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenThis affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids near the carboxyl terminus of human RNF25 protein.
Clone Name IsotypeIgG
Species Reactivityhuman, mouse Concentration1.0 mg/mL
Guaranteed Application *WB, IP Suggested DilutionsELISA: 1:10,000 - 1:40,000, WB: 1:200 - 1:2,000, IP: 1 mg
Buffer0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Note This antibody is suitable for Cancer, Immunology and Nuclear Signaling research. RING finger protein 25 (RNF25, also named AO7) contains a RING finger domain and is ubiquitously expressed in various tissues.  RNF25 was initially identified in a yeast two-hybrid screen of a murine T-cell library by using UbcH5b, an E2 enzyme, as bait.  RNF25 has also been shown to act as a putative E3 ligase, at least in vitro.  RNF25 localizes predominantly in the nucleus and supports the transcriptional activity of NF-kB by interacting with p65 in vivo upon stimulation with TNF.  Yeast two-hybrid data also suggest that RNF25 interacts with a number of other molecules which may be potential ubiquitin ligase substrates.  Among these are molecules that have critical roles in signal transduction and in regulation of translation (personal communication, Allan Weissman, CCR-NCI, Bethesda, MD).

Reference Data

Target NameHomo sapiens ring finger protein 25 (RNF25)
Alternative NameAO7
Database LinkNP_071898
Function
Related Pathway

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WB Image
Western blot using affinity purified anti-RNF25 antibody shows detection of RNF25 (arrow head) in HEK293 cells over-expressing human RNF25 (lane 1) or mouse RNF25 (lane 2). Lane 1 is a vector only control. The extracts were loaded onto a gel, followed by electrophoresis and transfer to nitrocellulose. The membrane was probed with the primary antibody diluted to 1:1,000. Personal Communication, Allan Weissman, CCR-NCI, Bethesda, MD.
IP Image
1 µg of Rockland’s affinity purified anti-RNF25 was used in immunoprecipitation of 20 µg HEK293 cell lysate over-expressing HA-tagged human RNF25 (lane 1) or vector only control (lane 2). The precipitated complex was loaded onto a gel, followed by electrophoresis and transfer to nitrocellulose. The membrane was probed with anti-HA tag antibody. Personal Communication, Allan Weissman, CCR-NCI, Bethesda, MD.

 

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