Home Antibody All anti-RFC4 antibodies
Also for RFC4 (NM_002916)
|Recombinant protein fragment contain a sequence corresponding to a region within amino acids 18 and 293 of RFC4|
|WB, IHC, IF
||WB:1:500-1:3000; IHC:1:100; IF:1:100-1:500;
|0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.|
|Purified by antigen-affinity chromatography. (Protein A or G Sepharose)
|Homo sapiens replication factor C (activator 1) 4, 37kDa (RFC4), transcript variant 1|
|The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kD. This gene encodes the 37 kD subunit. This subunit forms a core complex with the 36 and 40 kDa subunits. The core complex possesses DNA-dependent ATPase activity, which was found to be stimulated by PCNA in an in vitro system. Alternatively spliced transcript variants encoding the same protein have been reported. [provided by RefSeq]|
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Sample (30 ug of whole cell lysate) A: H1299 B: Hela C: HepG2 (anti-RFC4 antibody diluted at 1:1000)
Immunohistochemical analysis of paraffin-embedded MDAMB-468 Xenograft, using RFC4(anti-RFC4 antibody) antibody at 1:100 dilution.
Immunofluorescence analysis of paraformaldehyde-fixed A549, using RFC4(anti-RFC4 antibody) antibody at 1:200 dilution.