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Anti-RFC2 TRUEMAB Antibody clone 4E1
TrueMAB Antibodies - Made against Authentic Protein Antigens
Also for RFC2 (NM_002914)
|Human recombinant protei fragment corresponding to amino acids 1-234 of human RFC2(NP_002905) produced in E.coli.|
|| WB 1:200~2000, FLOW 1:100,
|PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography
|Homo sapiens replication factor C (activator 1) 2, 40kDa (RFC2), transcript variant 2|
Entrez Gene 5982 Human
|The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins, proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also called activator 1, is a protein complex consisting of five distinct subunits of 145, 40, 38, 37, and 36.5 kD. This gene encodes the 40 kD subunit, which has been shown to be responsible for binding ATP. Deletion of this gene has been associated with Williams syndrome. Alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008]. |
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RFC2 (RC201138, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RFC2.
Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-RFC2 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
HEK293T cells transfected with either RC201138 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-RFC2 antibody(TA504956), and then analyzed by flow cytometry.