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Home Antibody All anti-RFC2 antibodies

Anti-RFC2 TRUEMAB Antibody Clone OTI4E1

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA504956 RFC2 mouse monoclonal antibody, clone OTI4E1 (formerly 4E1) 100ul $325 In Stock
LC419017 RFC2 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF504956 Carrier-free (BSA/glycerol-free) RFC2 mouse monoclonal antibody, clone OTI4E1 (formerly 4E1) 100ug $450 3 Days
Conjugation is available for this antibody: choose conjugation type
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WB(2)
FC(1)

OriGene Data

ImmunogenHuman recombinant protei fragment corresponding to amino acids 1-234 of human RFC2(NP_002905) produced in E.coli.
Clone NameClone OTI4E1 IsotypeIgG1
Species ReactivityHuman Concentration1 mg/ml
Guaranteed Application *WB, FC Suggested Dilutions WB 1:200~2000, FLOW 1:100,
Predicted MW Explanation 35.1 kDa
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens replication factor C (activator 1) 2, 40kDa (RFC2), transcript variant 2
Alternative NameRFC40
Database LinkNP_002905
Entrez Gene 5982 Human
FunctionThe elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins, proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also called activator 1, is a protein complex consisting of five distinct subunits of 145, 40, 38, 37, and 36.5 kD. This gene encodes the 40 kD subunit, which has been shown to be responsible for binding ATP. Deletion of this gene has been associated with Williams syndrome. Alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008].
Related PathwayStem cell - PluripotencyDruggable Genome DNA replicationNucleotide excision repairMismatch repair

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RFC2 (RC201138, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RFC2.
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Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-RFC2 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
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HEK293T cells transfected with either RC201138 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-RFC2 antibody(TA504956), and then analyzed by flow cytometry.
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