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Anti-RELA PHOSPHO Antibody
Also for RELA (NM_021975)
|This NFKBp65 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S536 of human NFKBp65.|
||WB: 1:1000; IHC: 1:50-100
|Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. |
|This antibody is first purified by protein G affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
|Homo sapiens v-rel avian reticuloendotheliosis viral oncogene homolog A (RELA), transcript variant 1|
Delta-Notch Signaling Pathway
MAPK signaling pathway
Toll-like receptor signaling pathway
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Western blot analysis of Phospho-NFKBp65-S536 Antibody in human TNF alpha activated Hela cell line lysates. Phospho-RPS6KB1 (arrow) was detected using the purified PAb. (lane 1: without TNF alpha; lane 2: 10ng/ml TNF alpha, 5min)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.