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Anti-RELA Antibody EP2294Y
Also for RELA (NM_021975)
|A synthetic phospho-peptide corresponding to residues surrounding serine 536 of human NF-kappa-B protein.|
||Tissue culture supernatant
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:10000 - 1:20000; IP: 1:20
|Does not react with Mouse, Rat. Is unsuitable for Flow Cyt or ICC/IF.|
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
|Is unsuitable for Flow Cyt or ICC/IF.
|Homo sapiens v-rel avian reticuloendotheliosis viral oncogene homolog A (RELA), transcript variant 1|
|Nuclear factor B (NF-B) encompasses an important family of inducible transcriptional activators that regulate a wide variety of cellular and viral genes (1). The family members include p50, p52, p65 (RelA), c-Rel, and RelB. (2). The p65 subunit, similar to two others in the B family, RelB and c-Rel, contains two transactivation domains in the C-terminal region of the protein (3). Association with inhibitory proteins of the I?B family retains NF-?B in the cytoplasm. Degradation of IB proteins exposes the nuclear localization sequence (NLS), leading to nuclear translocation and subsequent binding of NF-B to DNA (4). p65 phosphorylation events occur in the cytoplasm or in the nucleus and are stimuli-specific and cell-type specific, on serine 276, 529, and 536 by various kinases, such as PKA, casein kinase II, IKK beta, and RSK1. Phosphorylation on these sites has been linked to regulation of NF-?B activation (5). |
Delta-Notch Signaling Pathway
MAPK signaling pathway
Toll-like receptor signaling pathway
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Western blot - NFkB p65 (phospho S536) antibody [EP2294Y]; All lanes : Anti-NF-kB p65 (phospho S536) antibody [EP2294Y] at 1/20000 dilution.Lane 1 : Untreated Daudi cell lysate.Lane 2 : Daudi cell lysate treated with Calyculin A + TNF-alpha.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit at 1/1000 dilution.Predicted band size : 65 kDa.