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Anti-REL Antibody EPR2558
|A synthetic peptide corresponding to residues in human c-Rel was used as an immunogen.|
||Tissue culture supernatant
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF
||ICC/IF: Use at an assay dependent concentration; WB: 1:1000 - 1:10000; IP: 1:10 - 1:100; IHC-P: 1:100 - 1:250; ICC: 1:100 - 1:250
|Does not react with Mouse, Rat. Is unsuitable for Flow Cyt.|
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05% (Protein A or G Sepharose)
|Is unsuitable for Flow Cyt.
|Homo sapiens v-rel avian reticuloendotheliosis viral oncogene homolog (REL)|
|c-Rel is a member of the nuclear factor kB (NF-kB) transcription factor family. The NF-kB family of dimeric transcription factors consists of four other mammalian members, RelA (p65), RelB, NF-kB1 (p105/p50) and NFkB2 (p100/p52) and is involved in many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. c-Rel is a proto-oncogene that may play a role in differentiation and lymphopoiesis. c-Rel forms different dimer combinations that act as transcriptional activators of repressors (1). Findings indicate that RelA and c-Rel control the development of the epidermis and associated appendages during embryogenesis; they regulate epidermal homeostasis in a postnatal environment through the suppression of innate immune-mediated inflammation (2).|
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Western blot - c-Rel antibody [EPR2558]; All lanes : Anti-c-Rel antibody [EPR2558] at 1/1000 dilution.Lane 1 : Daudi cell lysate.Lane 2 : HuT-78 cell lysate.Lane 3 : K562 cell lysate.Lysates/proteins at 10 µg per lane.Predicted band size : 69 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - c-Rel antibody [EPR2558]; Immunohistochemical analysis of Human diffuse B-cell lymphoma tissue using ab108299 at a dilution of 1/100.
Immunocytochemistry/ Immunofluorescence - Anti-c-Rel antibody [EPR2558]; ab108299 staining c-Rel in human macrophages by Immunocytochemistry/ Immunofluorescence.Cells were fixed in paraformaldehyde, permeabilized using Triton X-100, blocked with 5% goat serum for 1 hour at 37°C and then incubated with ab108299 at a 1/500 dilution for 3 hours at 37°C. The secondary used was an Alexa-Fluor 488 conjugated goat anti-rabbit monoclonal used at a 1/1000 dilution.