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Home Antibody All anti-RECQL4 antibodies
UltraMAB

Anti-RECQL4 GAT Antibody Polyclonal

Generated by Genomic Immunization

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Specifications Citations Customer Reviews Product Documents
SKU Description Amount Price Availability*  
TA590639 Rabbit Polyclonal RECQL4 Antibody 100ug $325 2 Weeks
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WB(2)
IHC(1)
IF(1)
ELISA(1)
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OriGene Data

ImmunogenDNA immunization. This antibody is specific for the N Terminus Region of the target protein.
Clone NamePolyclonal IsotypeIgG
Species ReactivityHuman Concentration1.27mg/ml
Guaranteed Application *WB, ELISA, IHC, IF Suggested Dilutions WB 1:5000~20000, IF 1:500, ELISA 1:100-1:2000
Buffer20 mM Potassium Phosphate, 150 mM Sodium Chloride, pH 7.0
Purification The fragment used for DNA immunization was expressed in E.coli and the purified protein fragment was used for affinity purification of the antibody.
Note This antibody was generated by SDIX's Genomic Antibody Technology ® (GAT). Learn about GAT

Reference Data

Target NameHomo sapiens RecQ like helicase 4 (RECQL4)
Alternative NameRECQ4
Database LinkNP_004251
Entrez Gene 9401 Human
FunctionRECQL4 is a DNA-dependent ATPase. May modulate chromosome segregation.
Related PathwayStem cell - PluripotencyDruggable Genome

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.


WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RECQL4 (RC215399, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RECQL4.
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WB Image
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-RECQL4 polyclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
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ELISA Image
ELISA: RECQL4 Antibody - Affinity Purified
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IHC Image
Immunohistochemistry: RECQL4 Antibody - Most of the normal tissues displayed strong nuclear positivity combined with slightly weaker cytoplasmic staining. Hepatocytes were weakly stained. Malignant tissues exhibited moderate nuclear immunoreactivity which occasionally was accompanied with cytoplasmic staining. Most malignant gliomas and cases of for example malignant carcinoids, lymphomas, colo-rectal and renal cancers showed strong staining. Image and statement courtesy of the Human Protein Atlas (HPA).
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IF Image
Immunofluorescent staining of HeLa cells using anti-RECQL4 rabbit polyclonal antibody (TA590639).
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