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Anti-RBP4 Antibody RB42
Also for RBP4 (NM_006744)
|Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with human recombinant RBP4.|
|PBS, pH 7.4 and 0.1% Sodium Azide|
|Protein A purified
|Homo sapiens retinol binding protein 4, plasma (RBP4)|
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Immunoreactivity of native free and native complexed RBP4 after several freeze-thaw cycles measured with the assay RB48 - RB42. Capture antibody: RB48 (1 ug/well) Detection antibody: RB42 labeled with stable Eu3+ chelate (0.2ug/well) Antigen: Native isolated RBP4.
Immunodetection of purified endogenous RBP4 in sandwich immunoassay by RB48-RB42 MAb assay in presence of 5 mM EDTA (blue line) or in absence of EDTA (pink line). Capture antibody: RB48 (1 ug/well) Detection antibody: RB42 labeled with stable Eu3+ chelate (0.2ug/well) Antigen: Native isolated RBP4
Immunodetection of RBP4 in Western blotting after SDSelectrophoresis in reducing conditions by MAb RB42 (lane 1), RB45 (lane 2), RB48 (lane 3) and RB51 (lane 4).