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Home Antibody All anti-RBL2 antibodies

Anti-RBL2 Antibody

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA319242
  • Rabbit polyclonal anti-p130 Rb2 antibody
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC417191) , 20ug Explanation
100ul 325 3-7 Days
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IHC(1)
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Also for RBL2 (NM_005611)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenRb2 (p130) peptide corresponding to a region near the C-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
Clone Name IsotypeIgG
Species Reactivityhuman, rat and mouse Concentration85 mg/mL
Guaranteed Application *IHC Suggested DilutionsELISA: 1:5,000 - 1:20,000, WB: 1:500 - 1:2,000, IHC: 1:200 - 1:1,000, IP: 1:100
Buffer0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Reference Data

Target NameHomo sapiens retinoblastoma-like 2 (RBL2)
Alternative NameP130; Rb2
Database LinkNP_005602
Entrez Gene 5934 Human
Entrez Gene 19651 Mouse
Entrez Gene 81758 Rat
Function
Related PathwayTranscription FactorsDruggable Genome Cell cycleTGF-beta signaling pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

IHC Image
Immunohistochemical staining of mouse tissue using anti-pRb2/p130 antiserum.  The staining shows the location of pRb2/p130 in developing mouse tissue.  Other detection systems should yield similar results. Sections were cut at 5-7 µm, mounted on glass and dried overnight at 37°C.  All sections were deparaffinized in xylene, rehydrated through a graded alcohol series and washed in phosphate-buffered saline (PBS).  PBS was used for all subsequent washes and for antiserum dilution.  Tissue sections were quenched sequentially in 0.5% hydrogen peroxide and blocked with diluted 10% normal goat anti-rabbit serum.  Slides were incubated at 20° C for 1 h with rabbit anti-pRb2/p130 (1:500) dilution, washed, and then reacted with diluted goat anti-rabbit biotinylated antibody for 30 min.  Slides were then reacted with streptavidin-peroxidase conjugate for 30 min at 20° C.  Diaminobenzidine was used as the final chromogen.  Negative controls for each tissue section were prepared by substituting the primary antiserum with pre-immune serum.

 

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