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Anti-RBL2 Antibody EP2387AY
Also for RBL2 (NM_005611)
|A synthetic phosphor peptide corresponding to residues surrounding serine 952 of human p130 was used as an immunogen.|
|Mouse, Human (Does not react with: Rat)
||Lot dependent; please refer to CoA along with shipment
||WB: 1: 10,000; IHC: 1: 100; ICC: 1: 100~250
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA
|Tissue culture supernatant
|Does not react with Rat
|Homo sapiens retinoblastoma-like 2 (RBL2)|
Entrez Gene 5934 Human
Entrez Gene 19651 Mouse
|p130 is a tumor suppressor of the pocket protein family whose expression is posttranscriptionally regulated and largely G0 restricted. The mechanism of down-regulation of p130 expression in proliferating cells was investigated and results indicate that the decline of p130 expression as G0 cells reenter the cell cycle is due to a decrease in protein stability. In vitro polyubiquitination of p130 by SCF(Skp2) was specifically dependent on phosphorylation of p130 on Serine 672 (1). The retinoblastoma family of proteins including pRB, p107 and p130 undergoes cell cycle dependent phosphorylation during the mid-G1 to S phase transition. This phosphorylation is dependent upon the activity of cyclin D/cdk4. In contrast to pRB and p107, p130 is phosphorylated during G0 and the early G1 phase of the cell cycle. Also it is thought that p130, forming a complex with Rad50 through RINT-1, blocks telomerase-independent telomere lengthening in normal cells. Data indicates complementary roles for the Rb/E2F pathway in the control of telomere length (3). |
|Transcription FactorsDruggable Genome Cell cycleTGF-beta signaling pathway|
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Western blot - Rb2 p130 (phospho S952) antibody [EP2387AY]; All lanes : Anti-Rb2 p130 (phospho S952) antibody [EP2387AY] at 1/500000 dilution.Lane 1 : Jurkat cell lysate, untreated.Lane 2 : Jurkat cell lysate, treated with AP.Lysates/proteins at 10 ug per lane.Secondary.HRP-labeled goat anti-mouse at 1/2000 dilution.Predicted band size : 128 kDa.Observed band size : 128 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rb2 p130 (phospho S952) antibody [EP2387AY]; Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using TA301132 at a dilution of 1/100.