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Anti-RBL2 Antibody EP2141Y
Also for RBL2 (NM_005611)
|A synthetic peptide corresponding to residues near the C-terminus of human Rb2 / p130 was used as an immunogen.|
|Human (Does not react with: Mouse, Rat)
||Lot dependent; please refer to CoA along with shipment
||WB: 1:500; IHC: 1:50 100; FC: 1:20
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant
|Does not react with Mouse, Rat
|Homo sapiens retinoblastoma-like 2 (RBL2)|
Entrez Gene 5934 Human
|p130 is a tumor suppressor of the pocket protein family whose expression is posttranscriptionally regulated and largely G0 restricted. The mechanism of down-regulation of p130 expression in proliferating cells was investigated and results indicate that the decline of p130 expression as G0 cells reenter the cell cycle is due to a decrease in protein stability. In vitro polyubiquitination of p130 by SCF(Skp2) was specifically dependent on phosphorylation of p130 on Serine 672 (1). The retinoblastoma family of proteins including pRB, p107 and p130 undergoes cell cycle dependent phosphorylation during the mid-G1 to S phase transition. This phosphorylation is dependent upon the activity of cyclin D/cdk4. In contrast to pRB and p107, p130 is phosphorylated during G0 and the early G1 phase of the cell cycle. Also it is thought that p130, forming a complex with Rad50 through RINT-1, blocks telomerase-independent telomere lengthening in normal cells. Data indicates complementary roles for the Rb/E2F pathway in the control of telomere length (3). |
|Transcription FactorsDruggable Genome Cell cycleTGF-beta signaling pathway|
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Western blot - p130 antibody [EP2141Y]; Anti-p130 antibody [EP2141Y] at 1/500 dilution + Jurkat cell lysate at 10 ug.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 128 kDa.Observed band size : 128 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - p130 antibody [EP2141Y]; Immunohistochemical staining of paraffin-embedded human breast carcinoma using TA301010 at 1/50 dilution.