A synthetic phospho-peptide corresponding to residues surrounding Thr356 of human Rb was used as immunogen. The antibody only detects Rb phosphorylated on Threonine 356.
Buffer
50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
The protein encoded by this gene is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in this gene are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma. [provided by RefSeq].
Related Pathway
Adipogenesis
Cell cycle
Senescence and Autophagy
TGF Beta Signaling Pathway
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Western blot analysis on Jurkat cell lysates using anti-Phospho-Rb (pT356) RabMAb (TA303685), 1:2000 dilution. Cells were either (A) untreated (B) treated with FBS
