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Home Antibody All anti-RB1 antibodies

Anti-RB1 PHOSPHO Antibody EPR2153AY

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA303685
  • Rabbit monoclonal antibody against Rb Phospho (pT356) (EPR2153AY ) (phospho-specific)
100ul $325 3-7 Days
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WB(1)
IHC(5)
IF(1)

OriGene Data

ImmunogenA synthetic phospho-peptide corresponding to residues surrounding Thr356 of human Rb was used as immunogen. The antibody only detects Rb phosphorylated on Threonine 356.
Clone NameEPR2153AY IsotypeIgG
Species ReactivityHuman (Does not react with: Mouse, Rat) ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, IF Suggested DilutionsWB: 1:2000; IP: 1:30; IHC-P: 1:100 - 1:250; ICC/IF: 1:100 - 1:250
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Note Is unsuitable for Flow Cyt.

Reference Data

Target NameHomo sapiens retinoblastoma 1 (RB1)
Alternative NameOSRC; p105-Rb; pp110; pRb; RB
Database LinkNP_000312
Entrez Gene 5925 Human
FunctionThe protein encoded by this gene is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in this gene are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma. [provided by RefSeq].
Related PathwayTranscription FactorsDruggable Genome Cell cyclePathways in cancerPancreatic cancerGliomaProstate cancerMelanomaMore Pathways >>

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

Western blot - Rb (phospho T356) antibody [EPR2153AY]; All lanes : Anti-Rb (phospho T356) antibody [EPR2153AY] at 1/2000 dilution.Lane 1 : Jurkat cell lysate.Lane 2 : Jurkat cell lysate treated with FBS.Lysates/proteins at 10 ug per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 106 kDa.Observed band size : 106 kDa.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rb (phospho T356) antibody [EPR2153AY]; Immunohistochemical analysis of paraffin-embedded human breast carcinoma using TA303685 at 1/100-1/250 dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Rb (phospho T356) antibody [EPR2153AY](TA303685); TA303685 showing positive staining in Lung adenocarcinoma tissue.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Rb (phospho T356) antibody [EPR2153AY](TA303685); TA303685 showing positive staining in Ovarian carcinoma tissue.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Rb (phospho T356) antibody [EPR2153AY](TA303685); TA303685 showing positive staining in Endometrial carcinoma tissue.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Rb (phospho T356) antibody [EPR2153AY](TA303685); TA303685 showing positive staining in Normal tonsil tissue.
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Immunocytochemistry - Rb (phospho T356) antibody [EPR2153AY]; Immunofluorescent staining of Hela cells using TA303685 at 1/100-1/250 dilution.
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