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Anti-RB1 PHOSPHO Antibody EP821Y
|A synthetic phospho-peptide corresponding to residues surrounding Thr373 of human Rb was used as immunogen. The antibody only detects Rb phosphorylated on Threonine 373.|
|Human (Does not react with: Mouse, Rat)
||Lot dependent; please refer to CoA along with shipment
||WB: 1:50,000; IHC: 1:2501:500; ICC: 1:50 100; IP: 1:30
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant
|Does not react with Mouse, Rat
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Western blot - Rb (phospho T373) antibody [EP821Y]; All lanes : Anti-Rb (phospho T373) antibody [EP821Y] at 1/50000 dilution.Lane 1 : Jurkat cell lysate (untreated).Lane 2 : Jurkat cell lysate (treated with FBS).Lysates/proteins at 10 ug per lane.Secondary.goat anti-rabbit HRP labelled at 1/2000 dilution.Predicted band size : 106 kDa.Observed band size : 110 kDa .
Immunohistochemistry (Paraffin-embedded sections) - Rb (phospho T373) antibody [EP821Y]; TA303684, at a 1/250 dilution, staining Human Rb (Phosphorylated) in Kidney carcinoma, using Immunohistochemistry, Paraffin Embedded tissue.