Home Antibody All anti-RASGRF1 antibodies
Anti-RASGRF1 GAT Antibody Polyclonal
Generated by Genomic Immunization
Also for RASGRF1 (NM_153815)
|DNA immunization. This antibody is specific for the C Terminus Region of the target protein.|
|Human , Monkey , Mouse
|WB, ELISA, IHC
|| WB 1:5000~20000, IHC 1:150,ELISA 1:100-1:2000
|20 mM Potassium Phosphate, 150 mM Sodium Chloride, pH 7.0|
|The fragment used for DNA immunization was expressed in E.coli and the purified protein fragment was used for affinity purification of the antibody.
|This antibody was generated by SDIX's Genomic Antibody Technology ® (GAT). Learn about GAT
|Homo sapiens Ras protein-specific guanine nucleotide-releasing factor 1 (RASGRF1), transcript variant 2|
|CDC25; CDC25L; GNRP; GRF1; GRF55; H-GRF55; PP13187; ras-GRF1|
Entrez Gene 5923 Human
Entrez Gene 19417 Mouse
|A critical step in signal transduction responses to stimulation of cell surfacereceptors by their ligands involves the accumulation of Ras proteins in theiractive GTP-bound state. To reach their active GTP-bound state, Ras proteinsmust first release bound GDP, a rate limiting step mediated by a guaninenucleotide releasing factor (GRF). The mammalian Ras p21 GRF protein hasbeen designated Ras-GRF1 p140. Ras-GRF1 accelerates release of GDP fromH- and N-Ras p21 protein in vitro, but not from the related Ral A or Cdc42HsGTP-binding proteins. Of interest, a region mapping within the amino terminaldomain of Ras-GRF1 is similar to both the human breakpoint cluster protein,Bcr, and the Dbl proto-oncogene product, a guanine nucleotide-releasingfactor for Cdc42Hs. Ras-GRF2 p135 has also been identified. Ras-GRF2p135 is highly homologous to Ras-GRF1 p140 except in the region betweenthe REM and CDC25 domains and appears to function similarly to Ras-GRF1p140.|
|Druggable Genome MAPK signaling pathwayFocal adhesion|
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RASGRF1 (RC215382, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RASGRF1.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-RASGRF1 polyclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-RASGRF1 rabbit polyclonal antibody. (TA590382)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-RASGRF1 rabbit polyclonal antibody. (TA590382)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-RASGRF1 rabbit polyclonal antibody. (TA590382)
Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-RASGRF1 rabbit polyclonal antibody. (TA590382)