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Home Antibody All anti-RASGRF1 antibodies
UltraMAB

Anti-RASGRF1 GAT Antibody Polyclonal

Generated by Genomic Immunization

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA590382
  • Rabbit Polyclonal RASGRF1 Antibody
100ug 325 In Stock
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WB(2)
IHC(4)
ELISA(1)
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Also for RASGRF1 (NM_153815)
cDNA Clone shRNA/siRNA Lysate Protein Request Antibody

OriGene Data

ImmunogenDNA immunization. This antibody is specific for the C Terminus Region of the target protein.
Clone NamePolyclonal IsotypeIgG
Species ReactivityHuman , Monkey , Mouse Concentration1.26mg/ml
Guaranteed Application *WB, ELISA, IHC Suggested Dilutions WB 1:5000~20000, IHC 1:150,ELISA 1:100-1:2000
Buffer20 mM Potassium Phosphate, 150 mM Sodium Chloride, pH 7.0
Purification The fragment used for DNA immunization was expressed in E.coli and the purified protein fragment was used for affinity purification of the antibody.
Note This antibody was generated by SDIX's Genomic Antibody Technology ® (GAT). Learn about GAT

Reference Data

Target NameHomo sapiens Ras protein-specific guanine nucleotide-releasing factor 1 (RASGRF1), transcript variant 2
Alternative NameCDC25; CDC25L; GNRP; GRF1; GRF55; H-GRF55; PP13187; ras-GRF1
Database LinkNP_722522
FunctionA critical step in signal transduction responses to stimulation of cell surfacereceptors by their ligands involves the accumulation of Ras proteins in theiractive GTP-bound state. To reach their active GTP-bound state, Ras proteinsmust first release bound GDP, a rate limiting step mediated by a guaninenucleotide releasing factor (GRF). The mammalian Ras p21 GRF protein hasbeen designated Ras-GRF1 p140. Ras-GRF1 accelerates release of GDP fromH- and N-Ras p21 protein in vitro, but not from the related Ral A or Cdc42HsGTP-binding proteins. Of interest, a region mapping within the amino terminaldomain of Ras-GRF1 is similar to both the human breakpoint cluster protein,Bcr, and the Dbl proto-oncogene product, a guanine nucleotide-releasingfactor for Cdc42Hs. Ras-GRF2 p135 has also been identified. Ras-GRF2p135 is highly homologous to Ras-GRF1 p140 except in the region betweenthe REM and CDC25 domains and appears to function similarly to Ras-GRF1p140.
Related Pathway
Focal Adhesion
MAPK signaling pathway
Signaling by GPCR

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RASGRF1 (RC215382, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RASGRF1.
WB Image
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-RASGRF1 polyclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
ELISA Image
ELISA: RASGRF1 Antibody
IHC Image
Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-RASGRF1 rabbit polyclonal antibody. (TA590382)
IHC Image
Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-RASGRF1 rabbit polyclonal antibody. (TA590382)
IHC Image
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-RASGRF1 rabbit polyclonal antibody. (TA590382)
IHC Image
Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-RASGRF1 rabbit polyclonal antibody. (TA590382)

 

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