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Anti-RANBP9 Antibody EP1353Y
Also for RANBP9 (NM_005493)
|A synthetic peptide corresponding to residues on the C-terminus of human RANBP9 was used as an immunogen.|
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, FC
||WB: 1:1000; IP: 1:20; FC: 1:1000; IHC-P: Use at an assay dependent concentration; ICC/IF: 1:100 - 1:250
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Does not react with Rat
|Homo sapiens RAN binding protein 9 (RANBP9)|
|BPM-L; BPM90; RanBP7; RANBPM|
|This gene encodes a protein that binds RAN, a small GTP binding protein belonging to the RAS superfamily that is essential for the translocation of RNA and proteins through the nuclear pore complex. The protein encoded by this gene has also been shown to interact with several other proteins, including met proto-oncogene, homeodomain interacting protein kinase 2, androgen receptor, and cyclin-dependent kinase 11. [provided by RefSeq]. |
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Western blot - RanBP9 antibody [EP1353Y]; Anti-Importin 9 antibody [EP1353Y] at 1/1000 dilution + Fetal brain lysate at 10 µg.Secondary.HRP-labelled goat anti-rabbit. at 1/2000 dilution.Predicted band size : 115 kDa.
Immunohistochemistry (Paraffin-embedded sections) - RanBP9 antibody [EP1353Y]; Immunohistochemical staining of paraffin-embedded human lymphoma using ab52605 at a dilution of 1/50.
Flow Cytometry - Anti-Importin 9 antibody [EP1353Y]; Overlay histogram showing HeLa cells stained with ab52605 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was Alexa Fluor? 488 goat anti-rabbit IgG (H+L) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1µg/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.