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Home Antibody All anti-RAD9A antibodies

Anti-RAD9A Antibody

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA302093
  • Rabbit Polyclonal Antibody against RAD9A
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC401451) , 20ug Explanation
100ug 325 3-7 Days
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WB(2)
IHC(1)
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Also for RAD9A (NM_004584)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenThis RAD9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from human RAD9.
Clone Name IsotypeRabbit Ig
Species ReactivityHuman, Mouse (Predicted: Monkey) Concentration0.25 mg/ml
Guaranteed Application *WB, IHC Suggested DilutionsWB: 1:1000, IHC: 1:50~100
Predicted MW Explanation 42489 Da
BufferPurified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Reference Data

Target NameHomo sapiens RAD9 homolog A (S. pombe) (RAD9A), transcript variant 1
Alternative NameRAD9
Database LinkNP_004575
Entrez Gene 5883 Human
Entrez Gene 19367 Mouse
Entrez Gene 712345 Monkey
FunctionThis gene product is highly similar to Schizosaccharomyces pombe rad9, a cell cycle checkpoint protein required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein is found to possess 3' to 5' exonuclease activity, which may contribute to its role in sensing and repairing DNA damage. It forms a checkpoint protein complex with RAD1 and HUS1. This complex is recruited by checkpoint protein RAD17 to the sites of DNA damage, which is thought to be important for triggering the checkpoint-signaling cascade. Use of alternative polyA sites has been noted for this gene.
Related PathwayStem cell - PluripotencyDruggable Genome

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot analysis of anti-Rad9 BH3 domain Pab (Cat. #TA302093) in HL-60 cell lysate. Rad9 BH3 domain (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
WB Image
Western blot analysis of Rad9(arrow) using rabbit polyclonal Rad9 BH3 domain Pab (Cat. #TA302093). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the Rad9 gene (Lane 2) (Origene Technologies).
IHC Image
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

 

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