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Anti-RAD17 PHOSPHO Antibody EPR3145


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SKU Description Amount Price Availability*  
  • Rabbit Monoclonal Antibody against RAD17 (Clone EPR3145) (Phospho-specific)
100ul 325 3-7 Days
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OriGene Data

ImmunogenA phospho-specific peptide corresponding to residues in human Rad17 was used as an immunogen. The antibody only detects Rad17 phosphoryated on Serine 656.
Clone NameEPR3145 IsotypeIgG
Species ReactivityHuman (Does not react with: Mouse, Rat) ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, IF Suggested DilutionsWB: 1:5,000~10,000; IHC: 1: 100~250; ICC: 1: 100~250
Predicted MW Explanation 77 kDa
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Does not react with Mouse, Rat

Reference Data

Target NameHomo sapiens RAD17 homolog (S. pombe) (RAD17), transcript variant 7
Alternative NameCCYC; HRAD17; R24L; RAD17SP; RAD24
Database LinkNP_579922
Entrez Gene 5884 Human
FunctionCheckpoint Rad proteins function early in the DNA damage signaling cascade to arrest cell cycle progression in response to DNA damage (1). There is a direct regulatory linkage between Rad17 and the checkpoint kinases, ATM and ATR (2). Rad17 has been reported to be a phosphorylation substrate of ATR and is critical for ATR-mediated checkpoint signaling and cell survival. Phosphorylation of Rad17 by ATR is important for genomic stability and restraint of S phase but is not essential for cell survival (3).
Related PathwayDruggable Genome

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Rad17 (phospho S656) antibody [EPR3145]; All lanes : Anti-Rad17 (phospho S656) antibody [EPR3145] at 1/10000 dilution.Lane 1 : HeLa cell lysate, untreated.Lane 2 : HeLa cell lysate treated with Calyculin A.Lysates/proteins at 10 ug per lane.Secondary.Goat anti-rabbit HRP at 1/2000 dilution.Predicted band size : 77 kDa.Observed band size : 80 kDa .
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rad17 (phospho S656) antibody [EPR3145]; TA301364, at 1/100-1/250 dilution, staining Rad17 in human breast carcinoma by immunohistochemistry using paraffin-embedded tissue.
IF Image
Immunocytochemistry/ Immunofluorescence - Rad17 (phospho S656) antibody [EPR3145]; TA301364, at 1/100-1/250 dilution, staining Rad17 in HeLa cells by immunofluorescence.


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