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Anti-RAD17 PHOSPHO Antibody EP1519Y


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SKU Description Amount Price Availability*  
  • Rabbit Monoclonal Antibody against RAD17 (Clone EP1519Y) (Phospho-specific)
100ul $325 3-7 Days
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OriGene Data

ImmunogenA phospho-specific peptide corresponding to residues surrounding Serine 645 of human Rad17 was used as immunogen. The antibody only detects Rad17 phosphorylated on Serine 645.
Clone NameEP1519Y IsotypeIgG
Species ReactivityHuman (Does not react with: Mouse, Rat) ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB Suggested DilutionsWB: 1:2500 - 1:5000
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Is unsuitable for Flow Cyt,ICC,IHC-P or IP.

Reference Data

Target NameHomo sapiens RAD17 homolog (S. pombe) (RAD17), transcript variant 7
Alternative NameCCYC; HRAD17; R24L; RAD17SP; RAD24
Database LinkNP_579922
Entrez Gene 5884 Human
FunctionCheckpoint Rad proteins function early in the DNA damage signaling cascade to arrest cell cycle progression in response to DNA damage (1). There is a direct regulatory linkage between the Rad17 homologue and the checkpoint kinases, ATM and ATR (2). ATR but not ATM phosphorylates the Rad17 checkpoint protein on Ser635 and Ser645 in vitro. In undamaged synchronized human cells, these two sites were phosphorylated in late G(1), S, and G(2)/M, but not in early-mid G(1). Treatment of cells with genotoxic stress induced phosphorylation of Rad17 in cells in early-mid G(1). This suggests that ATR and Rad17 are essential components of a DNA damage response pathway in mammalian cells (3).
Related PathwayDruggable Genome

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

Western blot - Anti-Rad17 (phospho S645) antibody [EP1519Y]; All lanes : Anti-Rad17 (phospho S645) antibody [EP1519Y] at 1/5000 dilution.Lane 1 : HeLa cell lysates, untreated.Lane 2 : HeLa cell lysates, treated with Lambda Phosphatase.Lysates/proteins at 10 ug per lane.Secondary.goat anti-rabbit HRP at 1/2000 dilution.Predicted band size : 77 kDa.Observed band size : 77 kDa.


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