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Anti-RACGAP1 Antibody EPR9018
Also for RACGAP1 (NM_001126103)
|A synthetic peptide corresponding to residues in human RACGAP1 was used as an immunogen.|
||Tissue culture supernatant
||Lot dependent; please refer to CoA along with shipment
||WB: 1:1000 - 1:10000; IHC-P: 1:50 - 1:100; ICC/IF: 1:100 - 1:250
|Does not react with Mouse, Rat. Is unsuitable for Flow Cyt or IP.|
|Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA
|Is unsuitable for Flow Cyt or IP.
|Homo sapiens Rac GTPase activating protein 1 (RACGAP1), transcript variant 2|
|CYK4; HsCYK-4; ID-GAP; MgcRacGAP|
|RACGAP1 belongs to the GTPase-activating protein (GAP) family. GAPs bind activated forms of Rho GTPases and stimulate GTP hydrolysis. Through this catalytic function, GAPs negatively regulate Rho-mediated signals. RACGAP1 plays a regulatory role in initiation of cytokinesis, controlling cell growth and differentiation of hematopoietic cells, regulating spermatogenesis, and in neuronal proliferation (1). |
Wnt Signaling Pathway
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Western blot - Anti-RACGAP1 antibody [EPR9018]; All lanes : Anti-RACGAP1 antibody [EPR9018] at 1/1000 dilution.Lane 1 : A431 cell lysate.Lane 2 : Raji cell lysate.Lane 3 : Jurkat cell lysate.Lane 4 : JAR cell lysate.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 71 kDa.Observed band size : 71 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RACGAP1 antibody [EPR9018]; Immunohistochemical analysis of paraffin-embedded Human testis tissue labelling RACGAP1 with TA310801 at 1/50 dilution.