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Home Antibody All anti-RAB27B antibodies
UltraMAB

Anti-RAB27B GAT Antibody Polyclonal

Generated by Genomic Immunization

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA590641
  • Rabbit Polyclonal RAB27B Antibody
100ug 325 In Stock
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WB(3)
IHC(2)
IF(2)
ELISA(1)
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Also for RAB27B (NM_004163)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenDNA immunization. This antibody is specific for the C Terminus Region of the target protein.
Clone NamePolyclonal IsotypeIgG
Species ReactivityHuman , Dog Concentration1mg/ml
Guaranteed Application *WB, ELISA, IHC, IF Suggested Dilutions WB 1:5000~20000, IF 1:500, ELISA 1:100-1:2000
Buffer20 mM Potassium Phosphate, 150 mM Sodium Chloride, pH 7.0
Purification The fragment used for DNA immunization was expressed in E.coli and the purified protein fragment was used for affinity purification of the antibody.
Note This antibody was generated by SDIX's Genomic Antibody Technology ® (GAT). Learn about GAT

Reference Data

Target NameHomo sapiens RAB27B, member RAS oncogene family (RAB27B)
Alternative NameC25KG
Database LinkNP_004154
FunctionRAB27B may be involved in targeting uroplakins to urothelial apical membranes (By similarity).
Related Pathway

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WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RAB27B (RC206519, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RAB27B.
WB Image
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-RAB27B polyclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
WB Image
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11; Lane 2: RT-4; Lane 3: U-251 MG; Lane 4: Human Plasma; Lane 5: Liver; Lane 6: TonsilThis validation was performed by Protein Atlas and the presentation of data is for informational purposes only.
ELISA Image
ELISA: RAB27B Antibody - Affinity Purified
IHC Image
Immunohistochemistry: RAB27B Antibody - Normal tissues displayed cytoplasmic staining which in most glandular epithelia and urothelial cells was combined with membranous positivity. Strong cytoplasmic immunoreactivity was for example observed in breast, salivary gland and prostate. Cells in CNS were negative. Malignancies in breast and prostate exhibited moderate to strong positivity. The malignant tissues were generally negative. Image and statement courtesy of the Human Protein Atlas (HPA).
IHC Image
Immunohistochemical staining of human urinary bladder shows strong cytoplasmic positivity in urothelial cells.This validation was performed by Protein Atlas and the presentation of data is for informational purposes only.
IF Image
Immunofluorescent staining of HeLa cells using anti-RAB27B rabbit polyclonal antibody (TA590641).
IF Image
Immunofluorescent staining of human cell line U-2 OS shows no positivity.This validation was performed by Protein Atlas and the presentation of data is for informational purposes only.

 

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