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Anti-PUM2 Antibody EPR3813
Also for PUM2 (NM_015317)
|A synthetic peptide corresponding to residues in human Pumilio-2 was used as an immunogen. This antibody is predicted to not cross react with Pumilio-1.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
||ICC: 1:100 - 250, IP: 1:10, WB: 1:5,000 - 10,000,
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Homo sapiens pumilio RNA-binding family member 2 (PUM2), transcript variant 1|
|Pumilio-2 is a sequence-specific RNA-binding protein that regulates translation and mRNA stability by binding the 3'-UTR of mRNA targets. It belongs to the PUF family, 84% identical to Pumilio-1. It is required to support proliferation and self-renewal of stem cells by regulating the translation of key transcripts (1). Pumilio-2 is highly expressed in testis and ovary and is predominately expressed in stem cells and germ cells. |
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Western blot - Pumilio 2 antibody [EPR3813]; All lanes : Anti-Pumilio 2 antibody [EPR3813] at 1/5000 dilution.Lane 1 : HeLa cell lysate.Lane 2 : 293T cell lysate.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 114 kDa.Observed band size : 114 kDa.