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Anti-PTPN1 GAT Antibody Polyclonal
Generated by Genomic Immunization
Also for PTPN1 (NM_002827)
|DNA immunization. In vivo generated recombinant protein fragment|
||WB 1:20000, IHC 1:150,ELISA 1:100-1:2000
|20 mM Potassium Phosphate, 150 mM Sodium Chloride, pH 7.0|
|The fragment used for DNA immunization was expressed in E.coli and the purified protein fragment was used for affinity purification of the antibody.
|This antibody was generated by SDIX's Genomic Antibody Technology ® (GAT). Learn about GAT
|Homo sapiens protein tyrosine phosphatase, non-receptor type 1 (PTPN1), transcript variant 1|
Entrez Gene 5770 Human
|The non-transmembrane protein tyrosine phosphatase, PTP1B, comprises 435 amino acids, of which the C-terminal 114 residues have been implicated in controlling both localization and function of this enzyme. Increased phosphorylation of PTP1B is seen to accompany the transition from G2 to M phase of the cell cycle. Immunofluorescence experiments indicate that PTP-1B is localized predominantly in the endoplasmic reticulum (ER). Subcellular fractionation is consistent with this localization and establishes that PTP-1B is tightly associated with microsomal membranes, with its phosphatase domain oriented towards the cytoplasm. The finding of a tyrosine phosphatase on the ER suggests new possibilities for cellular events controlled by tyrosine phosphorylation (2). It has also been shown that PTP1B is a novel substrate for Akt and that phosphorylation of PTP1B by Akt at Ser(50) may negatively modulate its phosphatase activity creating a positive feedback mechanism for insulin signaling (3).|
|TransmembranePhosphataseDruggable Genome Adherens junctionInsulin signaling pathway|
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PTPN1 (RC204902, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PTPN1.
Western Blot: - Samples: Lane 1, Marker: 250, 130, 95, 72, 55, 36, 28, 17, 11 Lane 2, RT-4 Lane 3, U-251MG sp Lane 4, Human Plasma Lane 5, Liver Lane 6, Tonsil, Target weight 50 kDa.
Immunohistochemical staining of paraffin-embedded Human liver tissue using anti-PTPN1 Rabbit Polyclonal antibody. (TA590672)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-PTPN1 Rabbit Polyclonal antibody. (TA590672)
Immunohistochemical staining of paraffin-embedded Human lymph node tissue using anti-PTPN1 Rabbit Polyclonal antibody. (TA590672)
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-PTPN1 Rabbit Polyclonal antibody. (TA590672)