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Home Antibody All anti-PTPN1 antibodies
UltraMAB

Anti-PTPN1 GAT Antibody Polyclonal

Generated by Genomic Immunization

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA590672
  • Rabbit Polyclonal PTPN1 Antibody
100ug 325 In Stock
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WB(2)
IHC(4)
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Also for PTPN1 (NM_002827)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenDNA immunization. In vivo generated recombinant protein fragment
Clone NamePolyclonal IsotypeIgG
Species ReactivityHuman Concentration0.8mg/ml
Guaranteed Application *WB, IHC Suggested Dilutions WB 1:20000, IHC 1:150,ELISA 1:100-1:2000
Buffer20 mM Potassium Phosphate, 150 mM Sodium Chloride, pH 7.0
Purification The fragment used for DNA immunization was expressed in E.coli and the purified protein fragment was used for affinity purification of the antibody.
Note This antibody was generated by SDIX's Genomic Antibody Technology ® (GAT). Learn about GAT

Reference Data

Target NameHomo sapiens protein tyrosine phosphatase, non-receptor type 1 (PTPN1), transcript variant 1
Alternative NamePTP1B
Database LinkNP_002818
Entrez Gene 5770 Human
FunctionThe non-transmembrane protein tyrosine phosphatase, PTP1B, comprises 435 amino acids, of which the C-terminal 114 residues have been implicated in controlling both localization and function of this enzyme. Increased phosphorylation of PTP1B is seen to accompany the transition from G2 to M phase of the cell cycle. Immunofluorescence experiments indicate that PTP-1B is localized predominantly in the endoplasmic reticulum (ER). Subcellular fractionation is consistent with this localization and establishes that PTP-1B is tightly associated with microsomal membranes, with its phosphatase domain oriented towards the cytoplasm. The finding of a tyrosine phosphatase on the ER suggests new possibilities for cellular events controlled by tyrosine phosphorylation (2). It has also been shown that PTP1B is a novel substrate for Akt and that phosphorylation of PTP1B by Akt at Ser(50) may negatively modulate its phosphatase activity creating a positive feedback mechanism for insulin signaling (3).
Related PathwayTransmembranePhosphataseDruggable Genome Adherens junctionInsulin signaling pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PTPN1 (RC204902, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PTPN1.
WB Image
Western Blot: - Samples: Lane 1, Marker: 250, 130, 95, 72, 55, 36, 28, 17, 11 Lane 2, RT-4 Lane 3, U-251MG sp Lane 4, Human Plasma Lane 5, Liver Lane 6, Tonsil, Target weight 50 kDa.
IHC Image
Immunohistochemical staining of paraffin-embedded Human liver tissue using anti-PTPN1 Rabbit Polyclonal antibody. (TA590672)
IHC Image
Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-PTPN1 Rabbit Polyclonal antibody. (TA590672)
IHC Image
Immunohistochemical staining of paraffin-embedded Human lymph node tissue using anti-PTPN1 Rabbit Polyclonal antibody. (TA590672)
IHC Image
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-PTPN1 Rabbit Polyclonal antibody. (TA590672)

 

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