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Home Antibody All anti-PTPN1 antibodies

Anti-PTPN1 Antibody EP1837Y

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA303674
  • Rabbit monoclonal antibody against PTP1B (EP1837Y )
  • FREE positive control: HEK293T cell transient overexpression lysate (LC419087) , 20ug
100ul $325 In Stock
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WB(1)
IHC(1)
IF(1)
FC(1)
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Also for PTPN1 (NM_002827)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues near the N-terminus of human PTP1B was used as an immunogen.
Clone NameEP1837Y IsotypeIgG
Species ReactivityHuman, Mouse, Rat Concentration0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsICC/IF: 1:100 - 1:250; WB: 1:10000 - 1:50000; IP: 1:80; FC: 1:15; IHC-P: Use at an assay dependent dilution
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant

Reference Data

Target NameHomo sapiens protein tyrosine phosphatase, non-receptor type 1 (PTPN1), transcript variant 1
Alternative NamePTP1B
Database LinkNP_002818
FunctionThe protein encoded by this gene is the founding member of the protein tyrosine phosphatase (PTP) family, which was isolated and identified based on its enzymatic activity and amino acid sequence. PTPs catalyze the hydrolysis of the phosphate monoesters specifically on tyrosine residues. Members of the PTP family share a highly conserved catalytic motif, which is essential for the catalytic activity. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP has been shown to act as a negative regulator of insulin signaling by dephosphorylating the phosphotryosine residues of insulin receptor kinase. This PTP was also reported to dephosphorylate epidermal growth factor receptor kinase, as well as JAK2 and TYK2 kinases, which implicated the role of this PTP in cell growth control, and cell response to interferon stimulation. [provided by RefSeq].
Related Pathway
Adherens junction
Hemostasis

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WB Image
Western blot - PTP1B antibody [EP1837Y]; Anti-PTP1B antibody [EP1837Y] at 1/200000 dilution + Jurkat cell lysate at 10 µg.Secondary.Goat anti-rabbit HRP labeled at 1/2000 dilution.Predicted band size : 50 kDa.Observed band size : 50 kDa.
IHC Image
Immunohistochemistry (Paraffin-embedded sections) - PTP1B antibody [EP1837Y]; Immunohistochemical staining of paraffin-embedded human tonsils using TA303674 at a 1/100 dilution.
IF Image
Immunocytochemistry/ Immunofluorescence - PTP1B antibody [EP1837Y]; ICC/IF image of TA303674 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody overnight at +4°C. The secondary antibody (green) was Alexa Fluor? 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor? 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
FC Image
Flow Cytometry-PTP1B antibody [EP1837Y](TA303674); Overlay histogram showing HepG2 cells stained with TA303674 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight? 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit monoclonal IgG (1µg/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 4% paraformaldehyde/permeabilized in 0.1% PBS-Tween used under the same conditions.

 

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