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Anti-PTK7 TrueMAB Antibody clone 2E7
TrueMAB Antibodies - Made against Authentic Protein Antigens
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Also for PTK7 (NM_152880)
|Full length human recombinant protein of human PTK7(NP_690619) produced in HEK293T cell.|
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IF, FC
|| WB 1:200~500, IF 1:100, FLOW 1:100,
|PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography (Protein A or G Sepharose)
|Homo sapiens protein tyrosine kinase 7 (PTK7), transcript variant PTK7-2|
|Receptor protein tyrosine kinases transduce extracellular signals across the cell membrane. A subgroup of these kinases lack detectable catalytic tyrosine kinase activity but retain roles in signal transduction. The protein encoded by this gene is a member of this subgroup of tyrosine kinases and may function as a cell adhesion molecule. This gene is thought to be expressed in colon carcinomas but not in normal colon, and therefore may be a marker for or may be involved in tumor progression. Four transcript variants encoding four different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]. |
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PTK7 (RC223416, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PTK7.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PTK7 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Anti-PTK7 mouse monoclonal antibody (TA503920) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PTK7(RC223416).
HEK293T cells transfected with either RC223416 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PTK7 antibody(TA503920), and then analyzed by flow cytometry.