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Home Antibody All anti-PTK2 antibodies

Anti-PTK2 PHOSPHO Antibody

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA324127
  • Anti-PTK2 (phospho-Tyr576/Tyr577) Rabbit Polyclonal Antibody
100ul 325 2-3 Weeks
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WB(1)
IF(1)
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Also for PTK2 (NM_153831)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenPeptide sequence around phosphorylation site of tyrosine 576/tyrosine 577 (S-T-Y(p)-Y(p)-K-A) derived from Human FAK.
Clone Name IsotypeIgG
Species ReactivityHuman Concentration1mg/ml
Guaranteed Application *WB, IF Suggested DilutionsWB: 1:500-1000, IF: 1:100-200
BufferPBS pH7.3, 0.05% NaN3, 50% glycerol
Purification Antigen affinity purification

Reference Data

Target NameHomo sapiens protein tyrosine kinase 2 (PTK2), transcript variant 1
Alternative NameFADK; FAK; FAK1; FRNK; p125FAK; pp125FAK; PPP1R71
Database LinkNP_722560
FunctionNon-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Plays a potential role in oncogenic transformations resulting in increased kinase activity.
Related Pathway
Focal Adhesion
Hemostasis

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WB Image
Predicted band size: 125 kDa . Positive control: HT29 cells untreated or treated with PMA lysate. Recommended dilution: 1/ 500-1000. (Gel: 8%SDS-PAGE Lane1: HT29 cells untreated with PMA lysate Lane2: HT29 cells treated with PMA lysate Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)
IF Image
Predicted cell location: Cytoplasm. Positive control: Hela cells. Recommended dilution: 1/ 100-200.The image is immunofluorescence of methanol-fixed Hela cells using PTK2 (phospho-Tyr576/Tyr577) antibody at dilution 1/100. (Original magnification: ×200)

 

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