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Anti-PTK2 Antibody EP841Y
Also for PTK2 (NM_153831)
|A synthetic peptide corresponding to residues surrounding tyrosine 861 of human FAK was used as an immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:10000 - 1:50000
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant
|Is unsuitable for Flow Cyt,ICC,IHC-P or IP.
|Homo sapiens protein tyrosine kinase 2 (PTK2), transcript variant 1|
|FADK; FAK; FAK1; FRNK; p125FAK; pp125FAK; PPP1R71|
|Focal adhesion kinase (FAK) is a non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis (1). FAK is composed of a central catalytic domain flanked by large N- and C-terminal regions. FAK is activated by phosphorylation at tyrosine 397 in response to integrin clustering which can be induced by cell adhesion or antibody cross-linking or via G-protein-coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid (2-3). Tyrosine 861 on FAK is a major Src phosphorylation site. Phospholyation on tyrosine 861 promotes FAK binding to Intergin (cytoplasmic tail) and leads to FAK mediated anti-apoptotic signaling pathway during hyperosmotic cell stress (4) Increased FAK expression has been correlated with the enhanced motility and invasiveness of human tumor cells, as well as with promoting increased cell proliferation (5). |
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Western blot - FAK (phospho Y861) antibody [EP841Y]; All lanes : Anti-FAK (phospho Y861) antibody [EP841Y].Lane 1 : Hela cell lysates, untreated.Lane 2 : Hela cell lysates treated with pervanadate.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti rabbit at 1/2000 dilution.Predicted band size : 119 kDa.Observed band size : 125 kDa .