Home Antibody All anti-PTCH1 antibodies
Anti-PTCH1 GAT Antibody Polyclonal
Generated by Genomic Immunization
Also for PTCH1 (NM_001083602)
|DNA immunization. This antibody is specific for the C Terminus Region of the target protein.|
|WB, ELISA, IHC
||WB: 1:500; ELISA: 1:100-1:2000; IHC: 1:500
|20 mM Potassium Phosphate, 150 mM Sodium Chloride, pH 7.0|
|The fragment used for DNA immunization was expressed in E.coli and the purified protein fragment was used for affinity purification of the antibody.
|This antibody was generated by SDIX's Genomic Antibody Technology ® (GAT). Learn about GAT
|Homo sapiens patched 1 (PTCH1), transcript variant 1a|
|BCNS; HPE7; NBCCS; PTC; PTC1; PTCH; PTCH11|
|This gene encodes a member of the patched gene family. The encoded protein is the receptor for sonic hedgehog, a secreted molecule implicated in the formation of embryonic structures and in tumorigenesis, as well as the desert hedgehog and indian hedgehog proteins. This gene functions as a tumor suppressor. Mutations of this gene have been associated with basal cell nevus syndrome, esophageal squamous cell carcinoma, trichoepitheliomas, transitional cell carcinomas of the bladder, as well as holoprosencephaly. Alternative splicing results in multiple transcript variants encoding different isoforms. Additional splice variants have been described, but their full length sequences and biological validity cannot be determined currently.|
Hedgehog signaling pathway
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Lane 1: Marker [kDa] 219, 111, 83, 48, 32, 26, 17; Lane 2: RT-4; Lane 3: U-251 MG; Lane 4: Human Plasma; Lane 5: Liver; Lane 6: TonsilThis validation was performed by Protein Atlas and the presentation of data is for informational purposes only.
Immunohistochemistry: Patched 1 Antibody - Normal and malignant tissues showed in general a moderate to strong cytoplasmic and membranous positivity. Malignant gliomas were mostly weakly stained or negative. Stroma and endothelial lining were distinctly stained in normal and tumor tissues. Image and statement courtesy of the Human Protein Atlas (HPA).
Immunohistochemistry: Patched 1 Antibody - With exception of malignant gliomas, malignant tissues such as in colo-rectal cancer showed in general a moderate to strong cytoplasmic and membranous positivity. Image was provided courtesy of the Swedish Human Proteome Resource (HPR) Center.
Immunohistochemical staining of human duodenum shows strong cytoplasmic positivity in glandular cells.This validation was performed by Protein Atlas and the presentation of data is for informational purposes only.