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Anti-PTBP1 Antibody EPR9048(B)
Also for PTBP1 (NM_002819)
|A synthetic peptide corresponding to residues in human PTBP1 was used as an immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF
||WB: 1:10000 - 1:50000; IHC-P: 1:100 - 1:250; ICC/IF: 1:250 - 1:500
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
|Tissue culture supernatant (Protein A or G Sepharose)
|Is unsuitable for Flow Cyt or IP.
|Homo sapiens polypyrimidine tract binding protein 1 (PTBP1), transcript variant 1|
|HNRNP-I; HNRNPI; HNRPI; pPTB; PTB; PTB-1; PTB-T; PTB2; PTB3; PTB4|
|PTBP1 belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA-binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. PTBP1 has four repeats of quasi-RNA recognition motif (RRM) domains that bind RNAs. It binds to the intronic polypyrimidine tracts that requires pre-mRNA splicing and acts via the protein degradation ubiquitin-proteasome pathway. It may also promote the binding of U2 snRNP to pre-mRNAs. PTBP1 is localized in the nucleoplasm and it is also detected in the perinucleolar structure (1).|
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Western blot - Anti-PTBP1 antibody [EPR9048(B)]; All lanes : Anti-PTBP1 antibody [EPR9048(B)] at 1/10000 dilution.Lane 1 : Human fetal liver lysate.Lane 2 : Jurkat cell lysate.Lane 3 : Daudi cell lysate.Lane 4 : A549 cell lysate.Lane 5 : BxPC3 cell lysate.Lysates/proteins at 10 µg per lane.Secondary.Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution.Predicted band size : 57 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTBP1 antibody [EPR9048(B)]; Immunohistochemical analysis of PTBP1 in paraffin embedded Human breast carcinoma tissue stained with ab133734 at a 1/100 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-PTBP1 antibody [EPR9048(B)]; Immunofluorescent staining of PTBP1 in A549 cells, using ab133734 at a 1/250 dilution.