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Home Antibody All anti-PSMD14 antibodies

Anti-PSMD14 Antibody EPR4257

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA307195
  • Rabbit monoclonal antibody against POH1(clone EPR4257)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC401764) , 20ug Explanation
100ul $325 3-7 Days
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WB(1)
IHC(1)
IF(1)
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Also for PSMD14 (NM_005805)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in human POH1 was used as an immunogen.
Clone NameEPR4257 IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, IF Suggested DilutionsWB: 1:1000 - 1:10000; IHC-P: 1:500 - 1:1000; ICC/IF: 1:500 - 1:1000
Predicted MW Explanation 35 kDa
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Is unsuitable for Flow Cyt or IP.

Reference Data

Target NameHomo sapiens proteasome (prosome, macropain) 26S subunit, non-ATPase, 14 (PSMD14)
Alternative NamePAD1; POH1; RPN11
Database LinkNP_005796
Entrez Gene 10213 Human
Entrez Gene 59029 Mouse
Entrez Gene 311078 Rat
FunctionPOH1, also called PSMD14, is a member of the peptidase M67A family and a metalloprotease component of the 26S proteasome that specifically cleaves the 'Lys-63'-linked polyubiquitin chains. The 26S proteasome is involved in the ATP-dependent degradation of ubiquitinated proteins. It is widely expressed with highest levels in the heart and skeletal muscle (1).
Related PathwayProteaseDruggable Genome Proteasome

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

Western blot - PSMD14 antibody [EPR4257]; All lanes : Anti-PSMD14 antibody [EPR4257] at 1/1000 dilution.Lane 1 : HepG2 cell lysates.Lane 2 : A431 cell lysates.Lane 3 : HeLa cell lysates.Lane 4 : Namalwa cell lysates.Lane 5 : K562 cell lysates.Lane 6 : MCF7 cell lysates.Lysates/proteins at 10 ug per lane.Secondary.HRP labelled Goat anti-Rabbit at 1/2000 dilution.Predicted band size : 35 kDa.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PSMD14 antibody [EPR4257]; TA307195, at 1/500 dilution staining PSMD14 in paraffin-embedded Human colon tissue, by Immunohistochemistry.
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Immunocytochemistry/ Immunofluorescence - PSMD14 antibody [EPR4257]; TA307195, at 1/500 dilution, staining PSMD14 in HeLa cells by Immunofluorescence.
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