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Anti-PSMD14 Antibody EPR4257
Also for PSMD14 (NM_005805)
|A synthetic peptide corresponding to residues in human POH1 was used as an immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF
||WB: 1:1000 - 1:10000; IHC-P: 1:500 - 1:1000; ICC/IF: 1:500 - 1:1000
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Is unsuitable for Flow Cyt or IP.
|Homo sapiens proteasome (prosome, macropain) 26S subunit, non-ATPase, 14 (PSMD14)|
|PAD1; POH1; RPN11|
|POH1, also called PSMD14, is a member of the peptidase M67A family and a metalloprotease component of the 26S proteasome that specifically cleaves the 'Lys-63'-linked polyubiquitin chains. The 26S proteasome is involved in the ATP-dependent degradation of ubiquitinated proteins. It is widely expressed with highest levels in the heart and skeletal muscle (1).|
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Western blot - PSMD14 antibody [EPR4257]; All lanes : Anti-PSMD14 antibody [EPR4257] at 1/1000 dilution.Lane 1 : HepG2 cell lysates.Lane 2 : A431 cell lysates.Lane 3 : HeLa cell lysates.Lane 4 : Namalwa cell lysates.Lane 5 : K562 cell lysates.Lane 6 : MCF7 cell lysates.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled Goat anti-Rabbit at 1/2000 dilution.Predicted band size : 35 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PSMD14 antibody [EPR4257]; ab109123, at 1/500 dilution staining PSMD14 in paraffin-embedded Human colon tissue, by Immunohistochemistry.
Immunocytochemistry/ Immunofluorescence - PSMD14 antibody [EPR4257]; ab109123, at 1/500 dilution, staining PSMD14 in HeLa cells by Immunofluorescence.