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Home Antibody All anti-PSMD10 antibodies

Anti-PSMD10 TRUEMAB Antibody Clone OTI3F6

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA500781 anti-PSMD10 mouse monoclonal antibody, clone OTI3F6 (formerly 3F6) 100ul $379 In Stock
LC419094 PSMD10 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF500781 Carrier-free (BSA/glycerol-free) PSMD10 mouse monoclonal antibody, clone OTI3F6 (formerly 3F6) 100ug $450 3-4 weeks
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WB(2)
IHC(12)
FC(2)
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Also for PSMD10 (NM_002814)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human PSMD10 (NP_002805) produced in HEK293T cell.
Clone NameClone OTI3F6 IsotypeIgG1
Species ReactivityHuman Concentration1 mg/ml
Guaranteed Application *WB, IHC, FC Suggested DilutionsWB 1:2000, IHC 1:50, Flow 1:100
Predicted MW Explanation 24.4 kDa
BufferPBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens proteasome (prosome, macropain) 26S subunit, non-ATPase, 10 (PSMD10), transcript variant 1
Alternative NamedJ889N15.2; p28; p28(GANK)
Database LinkNP_002805
Entrez Gene 5716 Human
FunctionThe 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits.Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a non-ATPase subunit of the 19S regulator. Two transcripts encoding different isoforms have been described. Pseudogenes have been identified on chromosomes 3 and 20.
Related Pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PSMD10 (RC202025, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PSMD10.
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PSMD10 monoclonal antibody.
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-PSMD10 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500781, Dilution 1:50)
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-PSMD10mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500781, Dilution 1:50)
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Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using anti-PSMD10mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500781, Dilution 1:50)
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Immunohistochemical staining of paraffin-embedded liver tissue within the normal limits using anti-PSMD10mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500781, Dilution 1:50)
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Immunohistochemical staining of paraffin-embedded Carcinoma of liver tissue using anti-PSMD10mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500781, Dilution 1:50)
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Immunohistochemical staining of paraffin-embedded Ovary tissue within the normal limits using anti-PSMD10mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500781, Dilution 1:50)
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-PSMD10mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500781, Dilution 1:50)
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Immunohistochemical staining of paraffin-embedded pancreas tissue within the normal limits using anti-PSMD10mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500781, Dilution 1:50)
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Immunohistochemical staining of paraffin-embedded endometrium tissue within the normal limits using anti-PSMD10mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500781, Dilution 1:50)
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of endometrium tissue using anti-PSMD10mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500781, Dilution 1:50)
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Immunohistochemical staining of paraffin-embedded prostate tissue within the normal limits using anti-PSMD10mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500781, Dilution 1:50)
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Immunohistochemical staining of paraffin-embedded Carcinoma of prostate tissue using anti-PSMD10mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500781, Dilution 1:50)
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Flow cytometric analysis of Hela cells, using anti-PSMD10 antibody(TA500781),(Red) compared to a nonspecific negative control antibody(TA50011)(Blue).
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Flow cytometric analysis of Jurkat cells, using anti-PSMD10 antibody(TA500781),(Red) compared to a nonspecific negative control antibody(TA50011)(Blue).
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