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Anti-PSEN2 Antibody EP1515Y
Also for PSEN2 (NM_000447)
|A synthetic peptide corresponding to residues on the C-terminus of human Presenilin 2 was used as an immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IP
||WB: 1:20000; IHC-P: Use at an assay dependent dilution; ICC/IF: 1:100 - 1:250; IP: 1:50
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Is unsuitable for Flow Cyt.
|Homo sapiens presenilin 2 (Alzheimer disease 4) (PSEN2), transcript variant 1|
|AD3L; AD4; CMD1V; PS2; STM2|
|Alzheimer's disease (AD) patients with an inherited form of the disease carry mutations in the presenilin proteins (PSEN1 or PSEN2) or the amyloid precursor protein (APP). These disease-linked mutations result in increased production of the longer form of amyloid-beta (main component of amyloid deposits found in AD brains). Presenilins are postulated to regulate APP processing through their effects on gamma-secretase, an enzyme that cleaves APP. Also, it is thought that the presenilins are involved in the cleavage of the Notch receptor such that, they either directly regulate gamma-secretase activity, or themselves act are protease enzymes. Two alternatively spliced transcript variants encoding different isoforms of PSEN2 have been identified. [provided by RefSeq]. |
Delta-Notch Signaling Pathway
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Western blot - Presenilin 2 antibody [EP1515Y]; Anti-Presenilin 2 antibody [EP1515Y] at 1/200000 dilution + PC-12 cell lysate at 10 µg.Secondary.Goat anti-rabbit HRP labeled at 1/2000 dilution.Predicted band size : 50 kDa.Observed band size : 23 kDa .
Western blot - Presenilin 2 antibody [EP1515Y]; Anti-Presenilin 2 antibody [EP1515Y] at 1/1000 dilution (for 16 hours at 4°C) + Human HeLa cells - whole cell lysate at 30 µg.Secondary.An HRP-conjugated Goat anti-rabbit IgG at 1/2000 dilution.developed using the ECL technique.Performed under reducing conditions.Predicted band size : 50 kDa.Observed band size : 24 kDa .Exposure time : 1 minuteThis image is courtesy of an anonymous Abreview
Immunohistochemistry (Paraffin-embedded sections) - Presenilin 2 antibody [EP1515Y]; Ab51249 (1:50) staining human Presenilin 2 in human kidney tissue by immunohistochemistry using paraffin embedded tissue.
Immunoprecipitation - Presenilin 2 antibody [EP1515Y]; ab51249 Immunoprecipitating Presenilin 2 in human HeLa whole cell lysate. 200µg of cell lysate was incubated with undiluted primary antibody and matrix (Protein G) for 1 hour at 4°C. For western blotting ab51249 (1/1000) was used to confirm successful immunoprecipation.