Homo sapiens protein kinase, DNA-activated, catalytic polypeptide (PRKDC), transcript variant 1
Synonyms:
DNA-PKcs; DNAPK; DNPK1; HYRC; HYRC1; p350; XRCC7
Immunogen
The antiserum was produced against synthesized phosphopeptide derived from humanDNA PKcs around the phosphorylation site of threonine 2609 (V-E-TP-Q-A).
Buffer
phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clone Name
Isotype
Species Reactivity
Human
Concentration
1ug/ul
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatography using non-phosphopeptide corresponding to the phosphorylation site. (Protein A or G Sepharose)
Guaranteed Application *
WB
Suggested Dilutions
WB: 1:500~1:1000
Background
Related Pathway
Cell cycle
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Western blot analysis of extracts from K562 cells untreated or treated with hydroxyurea, using DNAPKcs antibody (Lanes 1 and 2) and DNA PKcs (phospho-Thr2609) antibody (Lanes 3 and 4).
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