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Anti-PRKD2 PHOSPHO Antibody EP1496Y
Also for PRKD2 (NM_016457)
|A phosphor-synthetic peptide corresponding to residues surrounding Serine 876 was used as an immunogen. This antibody detects targets phosphorylated at Ser876.|
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1: 1000; IP: 1:40
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Does not react with Mouse, Rat
|Homo sapiens protein kinase D2 (PRKD2), transcript variant 1|
|HSPC187; nPKC-D2; PKD2|
|Protein kinases constitute a large superfamily of enzymes with key regulatory functions in nearly all signal transmission processes of eukaryotic cells; PKD2 is a novel phorbol ester- and growth factor-stimulated protein kinase (1). PKD2 is widely expressed in human and murine tissues and in cell lines such as HL60. In vivo, phorbol ester has been shown to autophosphorylate PKD2 in a synergistic fashion. Phosphorylation of Ser(876) of PKD2 correlated with the activation status of the kinase. The first zinc finger-like domain shares 88% identity to the corresponding domains in PKD/PKC-mu and PKC-nu (2). |
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Western blot - Protein Kinase D2 (phospho S876) antibody [EP1496Y]; All lanes : Anti-Protein Kinase D2 (phospho S876) antibody [EP1496Y] at 1/1000 dilution.Lane 1 : HeLa cell lysate, untreated.Lane 2 : HeLa cell lysate, treated with TPA.Lysates/proteins at 10 µg per lane.Secondary.Goat anti-Rabbit HRP labeled at 1/2000 dilution.Predicted band size : 97 kDa.Observed band size : 105 kDa .