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Home Antibody All anti-PRKD1 antibodies

Anti-PRKD1 Antibody EPR1492(2)

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA307410
  • Rabbit monoclonal antibody against PKC mu / PKD(clone EPR1492(2))
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC419136) , 20ug Explanation
100ul 325 3-7 Days
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Also for PRKD1 (NM_002742)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in human PKC mu / PKD was used as an immunogen.
Clone NameEPR1492(2) IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB Suggested DilutionsWB: 1:1000 - 1:10000
Predicted MW Explanation 102 kDa
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Is unsuitable for Flow Cyt,ICC,IHC-P or IP.

Reference Data

Target NameHomo sapiens protein kinase D1 (PRKD1)
Alternative NamePKC-MU; PKCM; PKD; PRKCM
Database LinkNP_002733
Entrez Gene 5587 Human
Entrez Gene 18760 Mouse
Entrez Gene 85421 Rat
FunctionPKC mu is a novel member of the subgroup of atypical protein kinase Cs (PKC). Deduced protein sequence shows strong homology to conserved domains of members of the PKC subfamily. In vitro phorbol ester binding studies and kinase assays with lysates of cells overexpressing PKC mu showed phorbol ester-independent kinase activity, autophosphorylation, and, in normal rat kidney (NRK) cells, predominant phosphorylation of a 30-kDa protein at serine residues. Data suggest a role of PKC mu in signal transduction pathways related to growth control (1). PKC mu is a cytosolic protein, which upon binding to the trans-Golgi network (TGN) regulates the fission of transport carriers specifically destined to the cell surface. Mutation of serines 744/748 to alanines in the activation loop of intact PKD inhibits its localization to the TGN. Moreover, anti-phospho-PKD antibody, which recognizes only the activated form of PKD, recognizes the TGN-bound PKD. Thus, activation of intact PKD is important for binding to the TGN (2). Results demonstrate that betagamma subunits of the heterotrimeric G proteins directly activates PKD by interacting with its pleckstrin homology domain (3).
Related PathwayProtein KinaseDruggable Genome

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - PKC mu antibody [EPR1492(2)]; All lanes : Anti-PKC mu antibody [EPR1492(2)] at 1/1000 dilution.Lane 1 : HeLa cell lysate.Lane 2 : Jurkat cell lysate.Lane 3 : HepG2 cell lysate.Lane 4 : K562 cell lysate.Lysates/proteins at 10 ug per lane.Predicted band size : 102 kDa.

 

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