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Anti-PRKD1 PHOSPHO Antibody EP1494Y
Also for PRKD1 (NM_002742)
|A phospho-specific peptide corresponding to residues surrounding S916 were used as an immunogen. This antibody detects PKC mu phosphorylated on Serine 916.|
||Tissue culture supernatant
|Human (Does not react with: Mouse, Rat)
||Lot dependent; please refer to CoA along with shipment
||WB: 1:50000 - 1:100000; IHC-P: 1:100 - 1:250; IP: 1:60
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant
|Is unsuitable for Flow Cyt or ICC.
|Homo sapiens protein kinase D1 (PRKD1)|
|PKC-MU; PKCM; PKD; PRKCM|
Entrez Gene 5587 Human
|PKC mu is a novel member of the subgroup of atypical protein kinase Cs (PKC). Deduced protein sequence shows strong homology to conserved domains of members of the PKC subfamily. In vitro phorbol ester binding studies and kinase assays with lysates of cells overexpressing PKC mu showed phorbol ester-independent kinase activity, autophosphorylation, and, in normal rat kidney (NRK) cells, predominant phosphorylation of a 30-kDa protein at serine residues. Data suggest a role of PKC mu in signal transduction pathways related to growth control (1). PKC mu is a cytosolic protein, which upon binding to the trans-Golgi network (TGN) regulates the fission of transport carriers specifically destined to the cell surface. (2). Results demonstrate that betagamma subunits of the heterotrimeric G proteins directly activates PKD by interacting with its pleckstrin homology domain (3). |
|Protein KinaseDruggable Genome |
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Western blot - Anti-PKC mu (phospho S916) antibody [EP1494Y]; .developed using the ECL technique.Performed under reducing conditions.Predicted band size : 102 kDa.Exposure time : 12 minutes
Western blot - PKC mu (phospho S916) antibody [EP1494Y]; All lanes : Anti-PKC mu (phospho S916) antibody [EP1494Y] at 1/100000 dilution.Lane 1 : lysate from untreated HeLa cells .Lane 2 : lyaste from HeLa cells treated with TPA.Lysates/proteins at 10 ug per lane.Secondary.HRP labelled Goat anti-Rabbit at 1/2000 dilution.Predicted band size : 102 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PKC mu (phospho S916) antibody [EP1494Y]; Immunohistochemistry analysis of paraffin-embedded Human placenta tissue using 1/100 TA300975.