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Anti-PRKCZ PHOSPHO Antibody
Also for PRKCZ (NM_002744)
|The antiserum was produced against synthesized phosphopeptide derived from human PKC ? around the phosphorylation site of threonine 560 (Q-L-TP-P-D).|
|Human: Thr560, Mouse: Thr560, Rat: Thr560
||WB: 1:500~1:3000, IHC: 1:50~1:100, ELISA: 1:20000
|Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.|
|The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. (Protein A or G Sepharose)
|PKC . (Phospho-Thr560) antibody detects endogenous levels of PKC . only when phosphorylated at threonine 560.
|Homo sapiens protein kinase C, zeta (PRKCZ), transcript variant 1|
EGFR1 Signaling Pathway
MAPK signaling pathway
Wnt Signaling Pathway
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Western blot analysis of extracts from COS7cells, treated with PMA (125ng/ml, 30mins), using PKC ? (Phospho-Thr560) antibody.The lane on the right is treated with the synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded human brain tissue, using PKC ? (Phospho-Thr560) antibody.The picture on the right is treated with the synthesized peptide.