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Home Antibody All anti-PRKCQ antibodies



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SKU Description Amount Price Availability*  
TA324172 Anti-PRKCQ (Phospho-Ser695) Rabbit Polyclonal Antibody 100ul 325
2-3 Weeks
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OriGene Data

ImmunogenPeptide sequence around phosphorylation site of serine 695 (N-F-S(p)-F-M) derived from Human PKC?.
Clone Name IsotypeIgG
Species ReactivityHuman; Mouse; Rat Concentration1mg/ml
Guaranteed Application *WB, IHC Suggested DilutionsWB: 1:500-1000, IHC: 1:50-100
Predicted MW Explanation 82kDa
BufferPBS pH7.3, 0.05% NaN3, 50% glycerol
Purification Antigen affinity purification

Reference Data

Target NameHomo sapiens protein kinase C, theta (PRKCQ), transcript variant 1
Alternative NamenPKC-theta; PRKCT
Database LinkNP_006248
Entrez Gene 5588 Human
Entrez Gene 18761 Mouse
Entrez Gene 85420 Rat
FunctionThis is a calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. Essential for T-cell receptor (TCR)-mediated T-cell activation, but is dispensable during TCR-dependent thymocyte development. Links the TCR signaling complex to the activation of NF-kappa-B in mature T lymphocytes. Required for interleukin-2 (IL2) production. PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.
Related PathwayProtein KinaseTranscription FactorsDruggable Genome Vascular smooth muscle contractionTight junctionT cell receptor signaling pathwayAdipocytokine signaling pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Predicted band size: 82 kDa . Positive control: 293 cells treated with PMA lysate. Recommended dilution: 1/ 500-1000. (Gel: 8%SDS-PAGE Lane1: 293 cells untreated with PMA lysate Lane2: 293 cells treated with PMA lysate Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)

IHC Image
Predicted cell location: Cytoplasm; Cell membrane. Positive control: Human lung carcinoma tissue. Recommended dilution: 1/ 50-100 The image on the left is immunohistochemistry of paraffin-embedded human lung carcinoma tissue using PRKCQ (Phospho-Ser695) antibody at dilution 1/50, on the right is treated with the synthetic peptide. (Original magnification:x200)


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