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Home Antibody All anti-PRKCE antibodies

Anti-PRKCE GAT Antibody Polyclonal

Generated by Genomic Immunization


Specifications Citations Customer Reviews Product Documents
SKU Description Amount Price Availability*  
TA590204 Rabbit Polyclonal PKC epsilon Antibody 100ug $325 In Stock
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Also for PRKCE (NM_005400)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenDNA immunization. This antibody is specific for the Middle Region of the target protein.
Clone NamePolyclonal IsotypeIgG
Species ReactivityHuman Concentration1.16934 mg/ml
Guaranteed Application *WB, ELISA Suggested Dilutions WB 1:20000,ELISA 1:100-1:2000
Buffer20 mM Potassium Phosphate, 150 mM Sodium Chloride, pH 7.0
Purification The fragment used for DNA immunization was expressed in E.coli and the purified protein fragment was used for affinity purification of the antibody.
Note This antibody was generated by SDIX's Genomic Antibody Technology ® (GAT). Learn about GAT

Reference Data

Target NameHomo sapiens protein kinase C, epsilon (PRKCE)
Alternative NamenPKC-epsilon; PKCE
Database LinkNP_005391
Entrez Gene 5581 Human
FunctionThe allergic reaction is mediated through the IgE high affinity receptor (FceRI). When a multivalent antigen is presented, a redistribution of the monomeric IgE FceRI receptor complexes cause the degranulation of mast cells and basophils, resulting in the subsequent release of factors responsible for the allergic reaction. The FceRI receptor is a tetrameric complex, consisting of a a-chain, b-chain and a dimeric g-chain. While the a-chain is primarily involved with the binding of IgE, the signal is transferred through the g subunit, which contains the immunoreceptor tyrosine activation motifs (ITAM) critical for initiating receptor mediated signal transduction through the Syk and Lyn tyrosine kinases. The FceRI receptor is able to engage and disengage the IgE, providing a versatile model for studying the function of kinase coupled receptors.
Related PathwayProtein KinaseDruggable Genome Vascular smooth muscle contractionTight junctionFc epsilon RI signaling pathwayFc gamma R-mediated phagocytosisType II diabetes mellitus

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PRKCE (RC217702, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PRKCE.
ELISA: PKC epsilon Antibody


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