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Anti-PRKCE TRUEMAB Antibody Clone OTI3A12
TrueMAB Antibodies - Made against Authentic Protein Antigens
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Also for PRKCE (NM_005400)
|Full length human recombinant protein of human PRKCE (NP_005391) produced in HEK293T cell.|
|WB, IHC, FC
||WB 1:500~2000, IHC 1:150, FLOW 1:100,
|PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography
|Homo sapiens protein kinase C, epsilon (PRKCE)|
Entrez Gene 5581 Human
|Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been shown to be involved in many different cellular functions, such as neuron channel activation, apoptosis, cardioprotection from ischemia, heat shock response, as well as insulin exocytosis. Knockout studies in mice suggest that this kinase is important for lipopolysaccharide (LPS)-mediated signaling in activated macrophages and may also play a role in controlling anxiety-like behavior. [provided by RefSeq]. |
|Protein KinaseDruggable Genome Vascular smooth muscle contractionTight junctionFc epsilon RI signaling pathwayFc gamma R-mediated phagocytosisType II diabetes mellitus|
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PRKCE (RC217702, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PRKCE.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PRKCE monoclonal antibody.
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-PRKCE mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502540)
HEK293T cells transfected with either RC217702 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PRKCE antibody(TA502540), and then analyzed by flow cytometry.
Flow cytometric Analysis of Hela cells, using anti-PRKCE antibody(TA502540),(Red), compared to a nonspecific negative control antibody,(Blue).
Flow cytometric Analysis of Jurkat cells, using anti-PRKCE antibody(TA502540),(Red), compared to a nonspecific negative control antibody,(Blue).