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Anti-PRKCD Antibody EP1486Y
Also for PRKCD (NM_212539)
|A synthetic peptide corresponding to residues surrounding serine 645 of human PKC delta was used as an immunogen.|
|Human (Does not react with: Mouse, Rat)
||Lot dependent; please refer to CoA along with shipment
|WB, IHC, IF
||WB: 1:10000 - 1:50000; IHC-P: 1:50 - 1:100; ICC/IF: 1:50 - 1:100
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Is unsuitable for Flow Cyt or IP.
|Homo sapiens protein kinase C, delta (PRKCD), transcript variant 2|
|ALPS3; CVID9; MAY1; nPKC-delta; PKCD|
Entrez Gene 5580 Human
|PKC delta has a biological activity that is closely related to Pkc1, and PKC delta activates the Pkc1-mediated pathway through activation of Bck1 kinase. PKC delta appears to play a critical role in the control of yeast and mammalian cell growth. Suppression experiments suggest that PKC delta desensitizes the pathway by regulating an aspect of G protein function (1). PKC delta phosphorylates hRad9 in vitro and in cells exposed to genotoxic agents. It has been shown that PKC delta is required for binding of hRad9 to Bcl-2. In concert with these results, inhibition of PKC delta attenuates Rad9-mediated apoptosis. These findings demonstrate that PKC delta is responsible for the regulation of Rad9 in the Hus1-Rad1 complex and in the apoptotic response to DNA damage (2). A mechanism for the regulation of peripheral B-cell survival by serine/threonine protein kinase C delta (PKC delta) is the spontaneous death of resting B cells, which is regulated by nuclear localization of PKC delta that contributes to phosphorylation of histone H2B at serine 14 (S14-H2B) (3).|
|Protein KinaseDruggable Genome Chemokine signaling pathwayVascular smooth muscle contractionTight junctionFc epsilon RI signaling pathwayFc gamma R-mediated phagocytosisNeurotrophin signaling pathwayMore Pathways >> |
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Western blot - PKC delta antibody [EP1486Y]; Anti-PKC delta antibody [EP1486Y] at 1/10000 dilution + 293T cell lysate at 10 ug.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 78 kDa.Observed band size : 78 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PKC delta antibody [EP1486Y]; Immunohistochemical analysis of PKC delta in paraffin embedded Human breast carcinoma tissue, using TA306969 at a 1/50 dilution.
Immunocytochemistry/ Immunofluorescence - PKC delta antibody [EP1486Y]; Immunofluorescent staining of PKC delta in HeLa cells, using TA306969 at a 1/50 dilution.